ArticleProceedings of the National Academy of Sciences of the United States of America2012
Direct observation of kinetic traps associated with structural transformations leading to multiple pathways of S-layer assembly.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed.
- A self-assembling surface layer flattens the cytokinetic furrow to aid cell division in an archaeon.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A suite of designed protein cages using machine learning and protein fragment-based protocols.Structure (London, England : 1993) · 2024Article
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- A Suite of Designed Protein Cages Using Machine Learning Algorithms and Protein Fragment-Based Protocols.bioRxiv : the preprint server for biology · 2023Article
- In vitro investigation of protein assembly by combined microscopy and infrared spectroscopy at the nanometer scale.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Rotational dynamics and transition mechanisms of surface-adsorbed proteins.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A New Method for Dispersing Pristine Carbon Nanotubes Using Regularly Arranged S-Layer Proteins.Nanomaterials (Basel, Switzerland) · 2021Article
- Two-step crystallization and solid-solid transitions in binary colloidal mixtures.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Weak Link with Actin Organizes Tight Junctions to Control Epithelial Permeability.Developmental cell · 2020Article
- A bacterial surface layer protein exploits multistep crystallization for rapid self-assembly.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Slp-coated liposomes for drug delivery and biomedical applications: potential and challenges.International journal of nanomedicine · 2019Review
- In-situ 2D bacterial crystal growth as a function of protein concentration: An atomic force microscopy study.Microscopy research and technique · 2018Article
- In vitro single-cell dissection revealing the interior structure of cable bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- In Vitro Characterization of the Two-Stage Non-Classical Reassembly Pathway of S-Layers.International journal of molecular sciences · 2017Article
- Impact of surface wettability on S-layer recrystallization: a real-time characterization by QCM-D.Beilstein journal of nanotechnology · 2017Article
- Analysis of self-assembly of S-layer protein slp-B53 from Lysinibacillus sphaericus.European biophysics journal : EBJ · 2017Article
- Ion-specific control of the self-assembly dynamics of a nanostructured protein lattice.ACS nano · 2015Article
- S-layers: principles and applications.FEMS microbiology reviews · 2014Article
- Self-assembled two-dimensional protein arrays in bionanotechnology: from S-layers to designed lattices.Current opinion in biotechnology · 2014Review
- Filming biomolecular processes by high-speed atomic force microscopy.Chemical reviews · 2014Review
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6 authors.
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Abstract
The concept of a folding funnel with kinetic traps describes folding of individual proteins. Using in situ Atomic Force Microscopy to investigate S-layer assembly on mica, we show this concept is equally valid during self-assembly of proteins into extended matrices. We find the S-layer-on-mica system possesses a kinetic trap associated with conformational differences between a long-lived transient state and the final stable state. Both ordered tetrameric states emerge from clusters of the monomer phase, however, they then track along two different pathways. One leads directly to the final low-energy state and the other to the kinetic trap. Over time, the trapped state transforms into the stable state. By analyzing the time and temperature dependencies of formation and transformation we find that the energy barriers to formation of the two states differ by only 0.7 kT, but once the high-energy state forms, the barrier to transformation to the low-energy state is 25 kT. Thus the transient state exhibits the characteristics of a kinetic trap in a folding funnel.
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