ArticleThe Journal of biological chemistry2012
CYP90A1/CPD, a brassinosteroid biosynthetic cytochrome P450 of Arabidopsis, catalyzes C-3 oxidation.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed, 205 citations in OpenAlex.
- Brassinosteroids as Central Regulators of Plant Growth, Stress Tolerance, and Agricultural Resilience.Plants (Basel, Switzerland) · 2026Review
- The functions of TaLAD1-A1, Rht8, and Rht-D1b in regulating leaf angle in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- A mechanistic framework for the recognition of chemically diverse brassinosteroids by BRI1-family receptor kinases.Nature plants · 2026Article
- Ubiquitination-Mediated Regulation of Brassinosteroid Signaling.Plants (Basel, Switzerland) · 2026Review
- Independent component analysis deciphers the genetic basis of cotton fiber elongation through eQTLs and sQTLs.Journal of advanced research · 2026Article
- Transcriptome Analysis Elucidates the Essential Pathways and Candidate Genes Involved in Chloroplast Development Between True Leaves and Cotyledon in Trichosanthes kirilowii Maxim.Biochemical genetics · 2026Article
- LncRNA7503 decreases peach (Prunus persica) branch number and angle by inducing pre-miR395a degradation and reducing bioactive BR content.Molecular horticulture · 2026Article
- Arabidopsis microtubule-BRI1-associated proteins negatively regulate hypocotyl elongation by controlling brassinosteroid-dependent cortical microtubule reorientation.Plant communications · 2026Article
- Manipulating brassinosteroid signaling pathway to genetically improve horticultural plants.aBIOTECH · 2025Review
- Induced variation in BRASSINOSTEROID INSENSITIVE 1 (BRI1) confers a compact wheat architecture.BMC plant biology · 2025Article
- A glycogen synthase kinase-3 gene enhances grain yield heterosis in semi-dwarf rapeseed.Plant molecular biology · 2025Article
- Genome-Wide Identification, Characterization, and Expression Analysis ofPlants (Basel, Switzerland) · 2025Article
- Genome-wide identification and expression analysis ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Maize-Tripsacum-Teosinte allopolyploid (MTP), a novel dwarf mutant inducer tool in maize.Plant biotechnology journal · 2025Article
- Genetic suppressor ofFrontiers in plant science · 2025Article
- Hormonal regulation and crosstalk during early endosperm and seed coat development.Plant reproduction · 2024Review
- Sex on Steroids: How Brassinosteroids Shape Reproductive Development in Flowering Plants.Plant & cell physiology · 2024Review
- Seed coat-derived brassinosteroid signaling regulates endosperm development.Nature communications · 2024Article
- Brassinosteroid biosynthesis and signaling: Conserved and diversified functions of core genes across multiple plant species.Plant communications · 2024Review
- A maize semi-dwarf mutant reveals a GRAS transcription factor involved in brassinosteroid signaling.Plant physiology · 2024Article
27 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brassinosteroids (BRs) are steroidal phytohormones that regulate plant growth and development. Whereas in Arabidopsis the network-like routes of BR biosynthesis have been elucidated in considerable detail, the roles of some of the biosynthetic enzymes and their participation in the different subpathways remained to be clarified. We investigated the function of the cytochrome P450 monooxygenase CYP90A1/CPD, which earlier had been proposed to act as a BR C-23 hydroxylase. Our GC-MS and genetic analyses demonstrated that the cpd mutation arrests BR synthesis upstream of the DET2-mediated 5α reduction step and that overexpression of the C-23 hydroxylase CYP90C1 does not alleviate BR deficiency in the cpd mutant. In line with these results, we found that CYP90A1/CPD heterologously expressed in a baculovirus-insect cell system catalyzes C-3 oxidation of the early BR intermediates (22S)-22-hydroxycampesterol and (22R,23R)-22,23-dihydroxycampesterol, as well as of 6-deoxocathasterone and 6-deoxoteasterone. Enzyme kinetic data of CYP90A1/CPD and DET2, together with those of the earlier studied CYP90B1, CYP90C1, and CYP90D1, suggest that BR biosynthesis proceeds mainly via the campestanol-independent pathway.
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