ArticleJournal of bacteriology2012
VirB-mediated positive feedback control of the virulence gene regulatory cascade of Shigella flexneri.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Virulence factors associated with pediatric shigellosis in Brazilian Amazon.BioMed research international · 2014Trial
- The read-through transcription-mediated autoactivation circuit for virulence regulator expression drives robust type III secretion system 2 expression in Vibrio parahaemolyticus.PLoS pathogens · 2024Article
- A Tale aboutMicroorganisms · 2023Review
- The T3SS ofMicroorganisms · 2020Review
- H-NS Family Members MvaT and MvaU Regulate the Pseudomonas aeruginosa Type III Secretion System.Journal of bacteriology · 2019Article
- Article
- Linalool, citral, eugenol and thymol: control of planktonic and sessile cells of Shigella flexneri.AMB Express · 2018Article
- MxiN Differentially Regulates Monomeric and Oligomeric Species of the Shigella Type Three Secretion System ATPase Spa47.Biochemistry · 2018Article
- Regulatory Hierarchies Controlling Virulence Gene Expression inFrontiers in microbiology · 2018Review
- The Galleria mellonella larvae as an in vivo model for evaluation of Shigella virulence.Gut microbes · 2017Article
- Review
- Article
- Riboregulators: Fine-Tuning Virulence in Shigella.Frontiers in cellular and infection microbiology · 2016Review
- Implications of Spatiotemporal Regulation of Shigella flexneri Type Three Secretion Activity on Effector Functions: Think Globally, Act Locally.Frontiers in cellular and infection microbiology · 2016Review
- Elongation factor P and modifying enzyme PoxA are necessary for virulence of Shigella flexneri.Infection and immunity · 2014Article
- Structural insights into VirB-DNA complexes reveal mechanism of transcriptional activation of virulence genes.Nucleic acids research · 2013Article
- Characterization of the ospZ promoter in Shigella flexneri and its regulation by VirB and H-NS.Journal of bacteriology · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shigella flexneri is a facultative intracellular pathogen that relies on a type III secretion system and its associated effector proteins to cause bacillary dysentery in humans. The genes that encode this virulence system are located on a 230-kbp plasmid and are transcribed in response to thermal, osmotic, and pH signals that are characteristic of the human lower gut. The virulence genes are organized within a regulatory cascade, and the nucleoid-associated protein H-NS represses each of the key promoters. Transcription derepression depends first on the VirF AraC-like transcription factor, a protein that antagonizes H-NS-mediated repression at the intermediate regulatory gene virB. The VirB protein in turn remodels the H-NS-DNA nucleoprotein complexes at the promoters of the genes encoding the type III secretion system and effector proteins, causing these genes to become derepressed. In this study, we show that the VirB protein also positively regulates the expression of its own gene (virB) via a cis-acting regulatory sequence. In addition, VirB positively regulates the gene coding for the VirF protein. This study reveals two hitherto uncharacterized feedback regulatory loops in the S. flexneri virulence cascade that provide a mechanism for the enhanced expression of the principal virulence regulatory genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.