ArticleJournal of bacteriology2012
Characterization of a new GlnR binding box in the promoter of amtB in Streptomyces coelicolor inferred a PhoP/GlnR competitive binding mechanism for transcriptional regulation of amtB.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
110 citing papers in PubMed.
- Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin inSynthetic and systems biotechnology · 2026Article
- Design of a Novel Diamine Biosensor to Guide the Engineering of Escherichia coli for Cadaverine Overproduction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The transcriptional repressor PheR regulates uptake and catabolism of phenanthrene in Sphingobium sp. SHPJ-2.The Journal of biological chemistry · 2026Article
- Regulation of DMSP organosulfur cycling in ubiquitous Roseobacter marine bacteria.The EMBO journal · 2026Article
- Novel regulatory mechanism of choline-O-sulfate and choline catabolism by two BetIs in Alphaproteobacteria.Applied and environmental microbiology · 2025Article
- Dual Role of MtHAC-1 in Regulating Cellulase and Xylanase Production in Myceliophthora thermophila.Microbial biotechnology · 2025Article
- A genetically encoded biosensor for point-of-care and live-cell detection of D-2-hydroxyglutarate.Nature communications · 2025Article
- The global transcriptional regulator MgaSpn affects the virulence of Streptococcus pneumoniae by regulating PcpA.BMC microbiology · 2025Article
- Defining the networks that connect RNase III and RNase J-mediated regulation of primary and specialized metabolism inJournal of bacteriology · 2025Article
- Structural insights into transcription regulation of the global OmpR/PhoB family regulator PhoP from Mycobacterium tuberculosis.Nature communications · 2025Article
- Positive regulation of a LuxR family protein, MilO, in mildiomycin biosynthesis.Applied and environmental microbiology · 2025Article
- Continuous carbon source supply is essential for high rifamycin productivity ofActa biochimica et biophysica Sinica · 2025Article
- Strain-specific strategies underlie convergent phosphate solubilization inISME communications · 2025Article
- DipR, a GntR/FadR-family transcriptional repressor: regulatory mechanism and widespread distribution of the dip cluster for dipicolinic acid catabolism in bacteria.Nucleic acids research · 2024Article
- The phytopathogenmLife · 2024Article
- H-NS involved in positive regulation of glycerol dehydratase gene expression inApplied and environmental microbiology · 2024Article
- The global nitrogen regulator GlnR is a direct transcriptional repressor of the key gluconeogenic geneJournal of bacteriology · 2024Article
- Pseudomonas aeruginosa regulator PvrA binds simultaneously to multiple pseudo-palindromic sites for efficient transcription activation.Science China. Life sciences · 2024Article
- SPD_0410 negatively regulates capsule polysaccharide synthesis and virulence inFrontiers in microbiology · 2024Article
- Regulation of the pleiotropic transcriptional regulator CodY on the conversion of branched-chain amino acids into branched-chain aldehydes inApplied and environmental microbiology · 2023Article
50 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcription of amtB in Streptomyces coelicolor has been proposed to be counter-regulated by GlnR (a global regulator for nitrogen metabolism) and PhoP (a global regulator for phosphate metabolism). However, the GlnR-protected region, which was deduced to be two 22-bp GlnR binding boxes (gTnAc-n6-GaAAc-n6-GtnAC-n6-GAAAc-n6, abbreviated as a1-b1 and a2-b2), was separated from the PhoP-protected region in the promoter of amtB, leaving the mechanism for this regulation undefined. In this study, another 22-bp GlnR binding box, which consisted of a3-site-n6-b3-site (a3-b3) overlapping with the PhoP-binding sequences, was identified in the promoter region of amtB by a DNase I footprinting assay. An electrophoretic mobility shift assay (EMSA) using purified recombinant GlnR and the synthetic amtB promoter fragments with the three GlnR binding boxes individually mutated demonstrated that every box was involved in GlnR binding in vitro. Further in vivo assays using the egfp reporter gene fused to various kinds of mutated promoter regions of amtB demonstrated that all of the three GlnR binding boxes were required for GlnR-mediated activation of amtB transcription under the nitrogen-limited condition. The results of EMSA using the amtB promoter with mixtures of recombinant His-tagged GlnR and Trx-His-S-tagged PhoP inferred that PhoP might compete against GlnR from binding at the a3-b3 site, attributable to the PhoP/GlnR counter-regulatory function subjected to further experimental proof.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.