ArticleMycopathologia2012
Rapid identification of Histoplasma capsulatum directly from cultures by multiplex PCR.
Article in Mycopathologia, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Accuracy of rapid diagnosis of Talaromyces marneffei: A systematic review and meta-analysis.PloS one · 2018Pooled it
- An integrated molecular approach for the detection and species-level identification ofMicrobiology spectrum · 2026Article
- Histoplasmosis With Diffuse Granulomatous Peritonitis in Crohn's Disease: A Mimic of Peritoneal Carcinomatosis.ACG case reports journal · 2022Article
- Recognition of Diagnostic Gaps for Laboratory Diagnosis of Fungal Diseases: Expert Opinion from the Fungal Diagnostics Laboratories Consortium (FDLC).Journal of clinical microbiology · 2021Article
- Frequency of the Mating-Type (MAT1) in Histoplasma capsulatum Isolates from Buenos Aires, Argentina.Mycopathologia · 2020Article
- Article
- Comparison of diagnostic methods to detect Histoplasma capsulatum in serum and blood samples from AIDS patients.PloS one · 2018Article
- Diagnostic Methods for Histoplasmosis: Focus on Endemic Countries with Variable Infrastructure Levels.Current tropical medicine reports · 2014Article
- Importance of the association of molecular and immunological diagnosis in immunocompetent patient with Histoplasma capsulatum and Cryptoccocus neoformans infection: a case report.The journal of venomous animals and toxins including tropical diseases · 2014Article
- Detection of environmental sources of Histoplasma capsulatum in Chiang Mai, Thailand, by nested PCR.Mycopathologia · 2013Article
- Endemic mycoses in immunocompromised hosts.Current infectious disease reports · 2013Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The multiplex PCR developed from a suspension of the yeast fungi correctly identified fifty-one clinical of H. capsulatum var. capsulatum strains isolated from clinical samples and soil specimens. The multiplex PCR was developed by combining two pairs of primers, one of them was specific to the H. capsulatum and the other one, universal for fungi, turned out to be specific to H. capsulatum, regardless of the fungus isolate studied. Primers designed to amplify a region of about 390-bp (Hc I-Hc II) and a region of approximately 600-bp (ITS1-ITS4) were used to identify a yeast isolated as H. capsulatum when both regions could be amplified. Absolute agreement (100 % sensitivity) could be shown between this assay and the cultures of H. capsulatum according to their morphological characteristics. Failure to amplify the target DNA sequence by PCR with primers Hc I-Hc II in the presence of the ITS1-ITS4 amplicon in isolates of P. brasiliensis, Cryptococcus neoformans, Trichosporon spp, Candida glabrata, C. albicans, C. tropicalis, C. parapsilosis, C. krusei, or Penicillium marneffei was an unequivocal sign of the high specificity of this assay. The assay specificity was also found to be 100 %. Incipient yeast forms obtained from clinical samples were identified as H. capsulatum by the PCR assay described before the morphological characteristics were registered shortening the time of diagnosis.
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