ArticleAnalytical and bioanalytical chemistry2012
Surface-enhanced Raman scattering (SERS) revealing chemical variation during biofilm formation: from initial attachment to mature biofilm.
Article in Analytical and bioanalytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 139 citations in OpenAlex.
- Development of surface enhanced Raman spectra coupled with machine learning analysis for differentiation of closely related species within Enterobacter cloacae complex.BMC microbiology · 2026Article
- Quantification of Antibiotic Diffusion in Biofilms Using Gold Nanostar Surface-enhanced Raman Spectroscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Biofilm-Associated Candidiasis: Pathogenesis, Prevalence, Challenges and Therapeutic Options.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Amphora coffeiformis extracellular polymeric substances and their potential applications in lead removal.Antonie van Leeuwenhoek · 2025Article
- Biomolecules in Pleistocene fossils from tropical cave indicate fossil biofilm.Scientific reports · 2024Article
- Environmental stressor assessment of hydrocarbonoclastic bacteria biofilms from a marine oil spill.Biotechnology reports (Amsterdam, Netherlands) · 2024Article
- Surface-enhanced Raman spectroscopy for the study of interaction of an antibacterial agent ([bis(1,3-dipentyl-1RSC advances · 2024Article
- Antibiofilm Property and Biocompatibility of Siloxane-Based Polymer Coatings Applied to Biomaterials.Materials (Basel, Switzerland) · 2023Article
- AC Electromagnetic Field Controls the Biofilms on the Glass Surface byMaterials (Basel, Switzerland) · 2023Article
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- The Determination, Monitoring, Molecular Mechanisms and Formation of Biofilm in E. coli.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Review
- Preparation of Laser-Ablated Ag Nanoparticle-MMT Clay-Based Beeswax Antibiofilm Coating.Antibiotics (Basel, Switzerland) · 2023Article
- In situ monitoring of Lentilactobacillus parabuchneri biofilm formation via real-time infrared spectroscopy.NPJ biofilms and microbiomes · 2022Article
- Proposal for Some Affordable Laboratory Biofilm Reactors and Their Critical Evaluations from Practical Viewpoints.Materials (Basel, Switzerland) · 2022Article
- Discrimination between Carbapenem-Resistant and Carbapenem-Sensitive Klebsiella pneumoniae Strains through Computational Analysis of Surface-Enhanced Raman Spectra: a Pilot Study.Microbiology spectrum · 2022Article
- Insights Into the Dynamics and Composition of Biofilm Formed by Environmental Isolate ofFrontiers in microbiology · 2022Article
- Epinephrine extensively changes the biofilm matrix composition inFrontiers in microbiology · 2022Article
- Review
- Comparative Analysis of Machine Learning Algorithms on Surface Enhanced Raman Spectra of ClinicalFrontiers in microbiology · 2021Article
- Mapping of a Subgingival Dual-Species Biofilm Model Using Confocal Raman Microscopy.Frontiers in microbiology · 2021Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Surface-enhanced Raman scattering (SERS) has recently been proved to be a promising technique for characterizing the chemical composition of the biofilm matrix. In the present study, to fully understand the chemical variations during biofilm formation, SERS based on silver colloidal nanoparticles was applied to evaluate the chemical components in the matrix of biofilm at different growth phases, including initial attached bacteria, colonies, and mature biofilm. Meanwhile, atomic force microscopy was also applied to study the changes of biofilm morphology. Three model bacteria, including Escherichia coli, Pseudomonas putida, and Bacillus subtilis, were used to cultivate biofilms. The results showed that the content of carbohydrates, proteins, and nucleic acids in the biofilm matrix increased significantly along with the biofilm growth of the three bacteria judging from the intensities and appearance probabilities of related marker peaks in the SERS spectra. The content of lipids, however, only increased in the Gram-negative biofilms (E. coli and P. putida) rather than the Gram-positive biofilm (B. subtilis). Our findings strongly suggest the SERS has significant potential for studying chemical variations during biofilm formation.
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