ArticleAnesthesiology2012
Roles of Gr-1+ leukocytes in postincisional nociceptive sensitization and inflammation.
Article in Anesthesiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Fucoidan as a Promising Drug for Pain Treatment: Systematic Review and Meta-Analysis.Marine drugs · 2024Pooled it
- Unique RNA Gene Expression Profile Is Seen in Chronic Non-Specific Low Back Pain.International journal of molecular sciences · 2025Article
- Exploring the role of neutrophils in inflammatory pain hypersensitivity via single-cell transcriptome profiling.Frontiers in immunology · 2025Article
- The MRGPR family of receptors in immunity.Immunity · 2024Review
- Neutrophil biology in injuries and diseases of the central and peripheral nervous systems.Progress in neurobiology · 2023Review
- The mechanisms and management of persistent postsurgical pain.Frontiers in pain research (Lausanne, Switzerland) · 2023Review
- Skin-resident dendritic cells mediate postoperative pain via CCR4 on sensory neurons.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Role of leukotriene B4 (LTB4)-LTB4 receptor 1 signaling in post-incisional nociceptive sensitization and local inflammation in mice.PloS one · 2022Article
- The "WWHow" Concept for Prospective Categorization of Post-operative Severity Assessment in Mice and Rats.Frontiers in veterinary science · 2022Article
- Longitudinal peripheral tissue RNA-Seq transcriptomic profiling, hyperalgesia, and wound healing in the rat plantar surgical incision model.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Investigating the Role of Ly6GFrontiers in pain research (Lausanne, Switzerland) · 2021Article
- AAAPT Diagnostic Criteria for Acute Knee Arthroplasty Pain.Pain medicine (Malden, Mass.) · 2020Article
- Patient and Surgery-Related Predictors of Acute Postoperative Pain.Current pain and headache reports · 2020Review
- Morphine immunomodulation prolongs inflammatory and postoperative pain while the novel analgesic ZH853 accelerates recovery and protects against latent sensitization.Journal of neuroinflammation · 2019Article
- Article
- γδ T Cells Modulate Myeloid Cell Recruitment but Not Pain During Peripheral Inflammation.Frontiers in immunology · 2019Article
- Divergent roles of immune cells and their mediators in pain.Rheumatology (Oxford, England) · 2018Review
- Sigma-1 receptors control immune-driven peripheral opioid analgesia during inflammation in mice.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Postoperative pain-from mechanisms to treatment.Pain reports · 2017Article
- CD11b+Ly6G- myeloid cells mediate mechanical inflammatory pain hypersensitivity.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
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Authors and funding
4 authors.
Funding
Abstract
backgroundNeutrophils are one of the predominant immune cells initially migrating to surgical wound edges. They produce mediators both associated with supporting (interleukin [IL]-1β, C5a) and reducing (opioid peptides) pain. Studies demonstrate neutrophil depletion/blockade reduces nociceptive sensitization after nerve injury and carrageenan administration, but enhance sensitization in complete Freund's adjuvant inflammation. This research identifies the contribution of infiltrating neutrophils to incisional pain and inflammation.
methodsAntibody-mediated Gr1 neutrophil depletion preceded hind paw incisions. Sensitization to mechanical and thermal stimuli, effects on edema and local levels of IL-1β and C5a were measured. Local effects of C5a or IL-1 receptor antagonists PMX-53 and anakinra on sensitization after neutrophil depletion were examined. Groups of 4-8 mice were used.
resultsAnti-Gr1 antibody depleted more than 90% of circulating and infiltrating skin neutrophils after incision. Neutrophil depletion did not change magnitude or duration of mechanical hypersensitivity in incised mice. However, paw edema was significantly reduced and heat hypersensitivity was slightly increased in depleted animals. In depleted animals IL-1β levels were half of controls 24 h after incision, whereas C5a levels were increased in both. Prominent IL-1β immunohistochemical staining of epidermis was seen in both groups. PMX-53 and anakinra reduced incisional mechanical and heat nociceptive sensitization to the same extent, regardless of neutrophil depletion.
conclusionsNeutrophil-derived IL-1β and C5a do not appear to contribute critically to peri-incisional nociceptive signaling. Other sources of mediators, such as epidermal cells, may need to be considered. Controlling inflammatory activation of resident cells in epidermis/deeper structures may show therapeutic efficacy in reducing pain from surgical incisions.
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