ArticleApplied and environmental microbiology2012
Effects of disinfection on Legionella spp., eukarya, and biofilms in a hot water system.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 64 citations in OpenAlex.
- Review
- The Contribution ofInternational journal of environmental research and public health · 2024Article
- Development of heat-shock resistance inApplied and environmental microbiology · 2023Article
- Compromised Effectiveness of Thermal Inactivation ofMicroorganisms · 2022Article
- Characterization of a Novel Regulator of Biofilm Formation in the PathogenBiomolecules · 2022Article
- Low Efficacy of Periodical Thermal Shock for Long-Term Control ofPathogens (Basel, Switzerland) · 2022Article
- Knowledge to Predict Pathogens:Microorganisms · 2022Review
- Combining Traditional and Molecular Techniques Supports the Discovery of a NovelFrontiers in microbiology · 2022Article
- Review
- Role of biofilms in the survival ofIranian journal of microbiology · 2021Article
- The Bacterial Community Diversity of Bathroom Hot Tap Water Was Significantly Lower Than That of Cold Tap and Shower Water.Frontiers in microbiology · 2021Article
- Intracellular Behaviour of ThreePathogens (Basel, Switzerland) · 2020Article
- Article
- Genetic and metabolic signatures of Salmonella enterica subsp. enterica associated with animal sources at the pangenomic scale.BMC genomics · 2019Article
- Advances inPathogens (Basel, Switzerland) · 2019Article
- AttenuatedBioMed research international · 2019Article
- The LetA/S two-component system regulates transcriptomic changes that are essential for the culturability of Legionella pneumophila in water.Scientific reports · 2018Article
- Effect of heat shock on hot water plumbing microbiota and Legionella pneumophila control.Microbiome · 2018Article
- Legionella confirmation in cooling tower water. Comparison of culture, real-time PCR and next generation sequencing.Saudi medical journal · 2018Article
- Factors Mediating Environmental Biofilm Formation byFrontiers in cellular and infection microbiology · 2018Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legionella species are frequently detected in hot water systems, attached to the surface as a biofilm. In this work, the dynamics of Legionella spp. and diverse bacteria and eukarya associated together in the biofilm, coming from a pilot scale 1 system simulating a real hot water system, were investigated throughout 6 months after two successive heat shock treatments followed by three successive chemical treatments. Community structure was assessed by a fingerprint technique, single-strand conformation polymorphism (SSCP). In addition, the diversity and dynamics of Legionella and eukarya were investigated by small-subunit (SSU) ribosomal cloning and sequencing. Our results showed that pathogenic Legionella species remained after the heat shock and chemical treatments (Legionella pneumophila and Legionella anisa, respectively). The biofilm was not removed, and the bacterial community structure was transitorily affected by the treatments. Moreover, several amoebae had been detected in the biofilm before treatments (Thecamoebae sp., Vannella sp., and Hartmanella vermiformis) and after the first heat shock treatment, but only H. vermiformis remained. However, another protozoan affiliated with Alveolata, which is known as a host cell for Legionella, dominated the eukaryal species after the second heat shock and chemical treatment tests. Therefore, effective Legionella disinfection may be dependent on the elimination of these important microbial components. We suggest that eradicating Legionella in hot water networks requires better study of bacterial and eukaryal species associated with Legionella in biofilms.
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