ArticleActa biomaterialia2012
Design and characterization of microporous hyaluronic acid hydrogels for in vitro gene transfer to mMSCs.
Article in Acta biomaterialia, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed.
- Synthetic biodegradable microporous hydrogels for in vitro 3D culture of functional human bone cell networks.Nature communications · 2024Article
- The comprehensive on-demand 3D bio-printing for composite reconstruction of mandibular defects.Maxillofacial plastic and reconstructive surgery · 2022Review
- In vivo restoration of dystrophin expression in mdx mice using intra-muscular and intra-arterial injections of hydrogel microsphere carriers of exon skipping antisense oligonucleotides.Cell death & disease · 2022Article
- Hydrogel-Based Localized Nonviral Gene Delivery in Regenerative Medicine Approaches-An Overview.Pharmaceutics · 2020Review
- Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral gene delivery.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- In Situ Forming Injectable Hydrogel For Encapsulation Of Nanoiguratimod And Sustained Release Of Therapeutics.International journal of nanomedicine · 2019Article
- It's All in the Delivery: Designing Hydrogels for Cell and Non-viral Gene Therapies.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Review
- Non-viral gene delivery systems for tissue repair and regeneration.Journal of translational medicine · 2018Review
- Biomaterials for Craniofacial Bone Regeneration.Dental clinics of North America · 2017Review
- Controlled and sustained delivery of siRNA/NPs from hydrogels expedites bone fracture healing.Biomaterials · 2017Article
- Injection of Microporous Annealing Particle (MAP) Hydrogels in the Stroke Cavity Reduces Gliosis and Inflammation and Promotes NPC Migration to the Lesion.Advanced materials (Deerfield Beach, Fla.) · 2017Article
- Engineered Hydrogels for Local and Sustained Delivery of RNA-Interference Therapies.Advanced healthcare materials · 2017Review
- Hydrogel-Based Controlled Delivery Systems for Articular Cartilage Repair.BioMed research international · 2016Article
- Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation.Acta biomaterialia · 2015Article
- Systematic evaluation of natural scaffolds in cutaneous wound healing.Journal of materials chemistry. B · 2015Article
- Porous hyaluronic acid hydrogels for localized nonviral DNA delivery in a diabetic wound healing model.Advanced healthcare materials · 2015Article
- A simple and efficient method for transfecting mouse embryonic stem cells using polyethylenimine.Experimental cell research · 2015Article
- Design of cell-matrix interactions in hyaluronic acid hydrogel scaffolds.Acta biomaterialia · 2014Review
- Non-viral DNA delivery from porous hyaluronic acid hydrogels in mice.Biomaterials · 2014Article
- Chemical sintering generates uniform porous hyaluronic acid hydrogels.Acta biomaterialia · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The effective and sustained delivery of DNA locally could increase the applicability of gene therapy in tissue regeneration and therapeutic angiogenesis. One promising approach is to use porous hydrogel scaffolds to encapsulate and deliver nucleotides in the form of nanoparticles to the affected sites. We have designed and characterized microporous (μ-pore) hyaluronic acid hydrogels which allow for effective cell seeding in vitro post-scaffold fabrication and allow for cell spreading and proliferation without requiring high levels of degradation. These factors, coupled with high loading efficiency of DNA polyplexes using a previously developed caged nanoparticle encapsulation (CnE) technique, then allowed for long-term sustained transfection and transgene expression of incorporated mMSCs. In this study, we examined the effect of pore size on gene transfer efficiency and the kinetics of transgene expression. For all investigated pore sizes (30, 60, and 100 μm), encapsulated DNA polyplexes were released steadily, starting by day 4 for up to 10 days. Likewise, transgene expression was sustained over this period, although significant differences between different pore sizes were not observed. Cell viability was also shown to remain high over time, even in the presence of high concentrations of DNA polyplexes. The knowledge acquired through this in vitro model can be utilized to design and better predict scaffold-mediated gene delivery for local gene therapy in an in vivo model where host cells infiltrate the scaffold over time.
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Registered trials
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