ArticleMolecular cell2012
Structural and mechanistic basis for the inhibition of Escherichia coli RNA polymerase by T7 Gp2.
Article in Molecular cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 47 citations in OpenAlex.
- RNA Management During T7 Infection.PHAGE (New Rochelle, N.Y.) · 2022Article
- Article
- Bacteriophage protein Gp46 is a cross-species inhibitor of nucleoid-associated HU proteins.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Bacteriophage Twort protein Gp168 is a β-clamp inhibitor by occupying the DNA sliding channel.Nucleic acids research · 2021Article
- Control of a programmed cell death pathway in Pseudomonas aeruginosa by an antiterminator.Nature communications · 2021Article
- Tunable expression rate control of a growth-decoupled T7 expression system by L-arabinose only.Microbial cell factories · 2021Article
- The Phage-EncodedViruses · 2020Article
- T7 phage factor required for managing RpoS inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Complete Genome Sequence of a Novel T7-Like Bacteriophage from a Pasteurella multocida Capsular Type A Isolate.Current microbiology · 2018Article
- Phenotypic consequences of RNA polymerase dysregulation in Escherichia coli.Nucleic acids research · 2017Article
- Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP.Nature communications · 2016Article
- The Xp10 Bacteriophage Protein P7 Inhibits Transcription by the Major and Major Variant Forms of the Host RNA Polymerase via a Common Mechanism.Journal of molecular biology · 2016Article
- The interaction of ω2 with the RNA polymerase β' subunit functions as an activation to repression switch.Nucleic acids research · 2015Article
- E. coli RNA Polymerase Determinants of Open Complex Lifetime and Structure.Journal of molecular biology · 2015Article
- Small things considered: the small accessory subunits of RNA polymerase in Gram-positive bacteria.FEMS microbiology reviews · 2015Review
- Structural biology of bacterial RNA polymerase.Biomolecules · 2015Review
- Coupling of downstream RNA polymerase-promoter interactions with formation of catalytically competent transcription initiation complex.Journal of molecular biology · 2014Article
- Trigger-helix folding pathway and SI3 mediate catalysis and hairpin-stabilized pausing by Escherichia coli RNA polymerase.Nucleic acids research · 2014Article
- ε, a new subunit of RNA polymerase found in gram-positive bacteria.Journal of bacteriology · 2014Article
- A bacteriophage transcription regulator inhibits bacterial transcription initiation by σ-factor displacement.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 4 countries.
Funding
Abstract
The T7 phage-encoded small protein Gp2 is a non-DNA-binding transcription factor that interacts with the jaw domain of the Escherichia coli (Ec) RNA polymerase (RNAp) β' subunit and inhibits transcriptionally proficient promoter-complex (RPo) formation. Here, we describe the high-resolution solution structure of the Gp2-Ec β' jaw domain complex and show that Gp2 and DNA compete for binding to the β' jaw domain. We reveal that efficient inhibition of RPo formation by Gp2 requires the amino-terminal σ(70) domain region 1.1 (R1.1), and that Gp2 antagonizes the obligatory movement of R1.1 during RPo formation. We demonstrate that Gp2 inhibits RPo formation not just by steric occlusion of the RNAp-DNA interaction but also through long-range antagonistic effects on RNAp-promoter interactions around the RNAp active center that likely occur due to repositioning of R1.1 by Gp2. The inhibition of Ec RNAp by Gp2 thus defines a previously uncharacterized mechanism by which bacterial transcription is regulated by a viral factor.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.