ArticleCell2012
Cell-cycle-coupled structural oscillation of centromeric nucleosomes in yeast.
Article in Cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 97 citations in OpenAlex.
- Cryo-EM structures of CENP-A nucleosomes isolated from human cells.Communications biology · 2026Article
- Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere.Science advances · 2023Article
- Nanoscale structural organization and stoichiometry of the budding yeast kinetochore.The Journal of cell biology · 2023Article
- A phylogenetically-restricted essential cell cycle progression factor in the human pathogen Candida albicans.Nature communications · 2022Article
- Orc4 spatiotemporally stabilizes centromeric chromatin.Genome research · 2021Article
- Phase-plate cryo-EM structure of the Widom 601 CENP-A nucleosome core particle reveals differential flexibility of the DNA ends.Nucleic acids research · 2020Article
- The ATAD2/ANCCA homolog Yta7 cooperates with Scm3Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Seeing is believing: our evolving view of kinetochore structure, composition, and assembly.Current opinion in cell biology · 2019Review
- The stoichiometry of the outer kinetochore is modulated by microtubule-proximal regulatory factors.The Journal of cell biology · 2019Article
- The Oligomerization Landscape of Histones.Biophysical journal · 2019Article
- The kinetochore module Okp1The EMBO journal · 2019Article
- Insights into Centromere DNA Bending Revealed by the Cryo-EM Structure of the Core Centromere Binding Factor 3 with Ndc10.Cell reports · 2018Article
- Quantifying Nucleation In Vivo Reveals the Physical Basis of Prion-like Phase Behavior.Molecular cell · 2018Article
- Quantitative mapping of fluorescently tagged cellular proteins using FCS-calibrated four-dimensional imaging.Nature protocols · 2018Article
- Article
- Structural plasticity of the living kinetochore.The Journal of cell biology · 2017Article
- Human centromeric CENP-A chromatin is a homotypic, octameric nucleosome at all cell cycle points.The Journal of cell biology · 2017Article
- An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites.eLife · 2017Article
- Promiscuous Histone Mis-Assembly Is Actively Prevented by Chaperones.Journal of the American Chemical Society · 2016Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The centromere is a specialized chromosomal structure that regulates chromosome segregation. Centromeres are marked by a histone H3 variant. In budding yeast, the histone H3 variant Cse4 is present in a single centromeric nucleosome. Experimental evidence supports several different models for the structure of centromeric nucleosomes. To investigate Cse4 copy number in live yeast, we developed a method coupling fluorescence correlation spectroscopy and calibrated imaging. We find that centromeric nucleosomes have one copy of Cse4 during most of the cell cycle, whereas two copies are detected at anaphase. The proposal of an anaphase-coupled structural change is supported by Cse4-Cse4 interactions, incorporation of Cse4, and the absence of Scm3 in anaphase. Nucleosome reconstitution and ChIP suggests both Cse4 structures contain H2A/H2B. The increase in Cse4 intensity and deposition at anaphase are also observed in Candida albicans. Our experimental evidence supports a cell-cycle-coupled oscillation of centromeric nucleosome structure in yeast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.