ArticleScientific reports2012
Ultraviolet shadowing of RNA can cause significant chemical damage in seconds.
Article in Scientific reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
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- Structures of a natural circularly permuted group II intron reveal mechanisms of branching and backsplicing.Nature structural & molecular biology · 2025Article
- Two riboswitch classes that share a common ligand-binding fold show major differences in the ability to accommodate mutations.Nucleic acids research · 2024Article
- Minimization of the E. coli ribosome, aided and optimized by community science.Nucleic acids research · 2024Article
- Quantitative probability estimation of light-induced inactivation of SARS-CoV-2.Scientific reports · 2024Article
- Full-Length NADBiochemistry · 2023Article
- Structure and function analysis of a type III preQThe Journal of biological chemistry · 2023Article
- Modular characterization of SARS-CoV-2 nucleocapsid protein domain functions in nucleocapsid-like assembly.Molecular biomedicine · 2023Article
- Chemical RNA Cross-Linking: Mechanisms, Computational Analysis, and Biological Applications.JACS Au · 2023Review
- General Strategies for RNA X-ray Crystallography.Molecules (Basel, Switzerland) · 2023Review
- Characterizing Fluorescence Properties of Turn-on RNA Aptamers.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Adoption of A-Z Junctions in RNAs by Binding of Zα Domains.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Synthesis of point-modified mRNA.Nucleic acids research · 2022Article
- Investigation of mouse hepatitis virus strain A59 inactivation under both ambient and cold environments reveals the mechanisms of infectivity reduction following UVC exposure.Journal of environmental chemical engineering · 2022Article
- Rational engineering enables co-crystallization and structural determination of the HIV-1 matrix-tRNA complex.STAR protocols · 2022Article
- A small RNA that cooperatively senses two stacked metabolites in one pocket for gene control.Nature communications · 2022Article
- Article
- Optimized photochemistry enables efficient analysis of dynamic RNA structuromes and interactomes in genetic and infectious diseases.Nature communications · 2021Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chemical purity of RNA samples is important for high-precision studies of RNA folding and catalytic behavior, but photodamage accrued during ultraviolet (UV) shadowing steps of sample preparation can reduce this purity. Here, we report the quantitation of UV-induced damage by using reverse transcription and single-nucleotide-resolution capillary electrophoresis. We found photolesions in a dozen natural and artificial RNAs; across multiple sequence contexts, dominantly at but not limited to pyrimidine doublets; and from multiple lamps recommended for UV shadowing. Irradiation time-courses revealed detectable damage within a few seconds of exposure for 254 nm lamps held at a distance of 5 to 10 cm from 0.5-mm thickness gels. Under these conditions, 200-nucleotide RNAs subjected to 20 seconds of UV shadowing incurred damage to 16-27% of molecules; and, due to a 'skin effect', the molecule-by-molecule distribution of lesions gave 4-fold higher variance than a Poisson distribution. Thicker gels, longer wavelength lamps, and shorter exposure times reduced but did not eliminate damage. These results suggest that RNA biophysical studies should report precautions taken to avoid artifactual heterogeneity from UV shadowing.
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