ArticlePloS one2012
Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Review
- A red-green photochromic bacterial protein as a new contrast agent for improved photoacoustic imaging.Photoacoustics · 2022Article
- Over the rainbow: structural characterization of the chromoproteins gfasPurple, amilCP, spisPink and eforRed.Acta crystallographica. Section D, Structural biology · 2022Article
- Structure-based analysis and evolution of a monomerized red-colored chromoprotein from the Olindias formosa jellyfish.Protein science : a publication of the Protein Society · 2022Article
- Optogenetic Imaging of Protein Activity Using Two-Photon Fluorescence Lifetime Imaging Microscopy.Advances in experimental medicine and biology · 2021Review
- Fluorescent Biosensors for Neuronal Metabolism and the Challenges of Quantitation.Current opinion in neurobiology · 2020Review
- Fusion proteins with chromogenic and keratin binding modules.Scientific reports · 2019Article
- ShadowR: a novel chromoprotein with reduced non-specific binding and improved expression in living cells.Scientific reports · 2019Article
- Design of a chromogenic substrate for elastase based on split GFP system-Proof of concept for colour switch sensors.Biotechnology reports (Amsterdam, Netherlands) · 2019Article
- Photoswitching FRET to monitor protein-protein interactions.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement.Scientific reports · 2017Article
- Dual observation of the ATP-evoked small GTPase activation and CaScientific reports · 2016Article
- Engineering Dark Chromoprotein Reporters for Photoacoustic Microscopy and FRET Imaging.Scientific reports · 2016Article
- Advances in Imaging Techniques and Genetically Encoded Probes for Photoacoustic Imaging.Theranostics · 2016Review
- A dark green fluorescent protein as an acceptor for measurement of Förster resonance energy transfer.Scientific reports · 2015Article
- X-Ray Crystal Structure and Properties of Phanta, a Weakly Fluorescent Photochromic GFP-Like Protein.PloS one · 2015Article
- Engineering genetically encoded FRET sensors.Sensors (Basel, Switzerland) · 2014Article
- Fluorescence lifetime imaging microscopy in the medical sciences.Protoplasma · 2014Review
- Phanta: a non-fluorescent photochromic acceptor for pcFRET.PloS one · 2013Article
- A green fluorescent protein containing a QFG tri-peptide chromophore: optical properties and X-ray crystal structure.PloS one · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; ε(585 nm), 64,000 M(-1) x cm(-1)) for use as a Förster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potential to increase the number of cellular parameters that can be imaged simultaneously.
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Registered trials
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