ArticlePloS one2012
Identification and characterization of the cognate anti-sigma factor and specific promoter elements of a T. tengcongensis ECF sigma factor.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Studies of the extracytoplasmic function sigma factor PG0162 in Porphyromonas gingivalis.Molecular oral microbiology · 2016Article
- Global mapping transcriptional start sites revealed both transcriptional and post-transcriptional regulation of cold adaptation in the methanogenic archaeon Methanolobus psychrophilus.Scientific reports · 2015Article
- Dual positive feedback regulation of protein degradation of an extra-cytoplasmic function σ factor for cell differentiation in Streptomyces coelicolor.The Journal of biological chemistry · 2013Article
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Authors and funding
6 authors.
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Abstract
Extracytoplasmic function (ECF) σ factors, the largest group of alternative σ factors, play important roles in response to environmental stresses. Tt-RpoE1 is annotated as an ECF σ factor in Thermoanaerobacter tengcongensis. In this study, we revealed that the Tt-tolB gene located downstream of the Tt-rpoE1 gene encoded the cognate anti-σ factor, which could inhibit the transcription activity of Tt-RpoE1 by direct interaction with Tt-RpoE1 via its N-terminal domain. By in vitro transcription assay, the auto-regulation ability of Tt-RpoE1 was determined, and band shift assay showed that Tt-RpoE1 preferred to bind a fork-junction promoter DNA. With truncation or base-specific scanning mutations, the contribution of the nucleotides in -35 and -10 regions to interaction between Tt-RpoE1 and promoter DNA was explored. The promoter recognition pattern of Tt-RpoE1 was determined as 5' tGTTACN(16)CGTC 3', which was further confirmed by in vitro transcription assays. This result showed that the Tt-RpoE1-recognized promoter possessed a distinct -10 motif (-13CGTC-10) as the recognition determinant, which is distinguished from the -10 element recognized by σ(70). Site-directed mutagenesis in Region 2.4 of Tt-RpoE1 indicated that the "D" residue of DXXR motif was responsible for recognizing the -12G nucleotide. Our results suggested that distinct -10 motif may be an efficient and general strategy used by ECF σ factors in adaptive response regulation of the related genes.
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