ArticlePloS one2012
High-throughput multilocus sequence typing: bringing molecular typing to the next level.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Methicillin-ResistantToxins · 2020Pooled it
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- Helicobacter pylori in oral cavity: current knowledge.Clinical and experimental medicine · 2024Review
- Diagnostic Methods for Helicobacter pylori.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2024Review
- Diversity of Endodontic Enterococcus faecalis from Brazil with a High Occurrence of the ST 397 Clone.Brazilian dental journal · 2024Article
- Serratia marcescens Colonization in a Neonatal Intensive Care Unit Has Multiple Sources, with Sink Drains as a Major Reservoir.Applied and environmental microbiology · 2023Article
- Comparison of four multilocus sequence typing schemes and amino acid biosynthesis based on genomic analysis of Bacillus subtilis.PloS one · 2023Article
- Discrimination ofJournal of microbiology and biotechnology · 2022Article
- New-Generation Sequencing Technology in Diagnosis of Fungal Plant Pathogens: A Dream Comes True?Journal of fungi (Basel, Switzerland) · 2022Review
- A High-Throughput Short Sequence Typing Scheme for Serratia marcescens Pure Culture and Environmental DNA.Applied and environmental microbiology · 2021Article
- Occurrence and distribution of fecal indicators and pathogenic bacteria in seawater and Perna perna mussel in the Gulf of Annaba (Southern Mediterranean).Environmental science and pollution research international · 2021Article
- Clonal evolution ofJournal of oral microbiology · 2021Article
- Microbial source tracking using metagenomics and other new technologies.Journal of microbiology (Seoul, Korea) · 2021Review
- Successful High-Dosage Monotherapy of Tigecycline in a Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2020Article
- nanoMLST: accurate multilocus sequence typing using Oxford Nanopore Technologies MinION with a dual-barcode approach to multiplex large numbers of samples.Microbial genomics · 2020Article
- Testing the "Grandma Hypothesis": Characterizing Skin Microbiome Diversity as a Project-Based Learning Approach to Genomics.Journal of microbiology & biology education · 2020Article
- Potential group B Streptococcus interspecies transmission between cattle and people in Colombian dairy farms.Scientific reports · 2019Article
- Phylogenetic and Molecular Profile ofMicroorganisms · 2019Article
- Antimicrobial Resistance in Commensal Escherichia coli Isolated from Pigs and Pork Derived from Farms Either Routinely Using or Not Using In-Feed Antimicrobials.Microbial drug resistance (Larchmont, N.Y.) · 2018Article
- Microbial sequence typing in the genomic era.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2018Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multilocus sequence typing (MLST) is a widely used system for typing microorganisms by sequence analysis of housekeeping genes. The main advantage of MLST in comparison to other typing techniques is the unambiguity and transferability of sequence data. However, a main disadvantage is the high cost of DNA sequencing. Here we introduce a high-throughput MLST (HiMLST) method that employs next-generation sequencing (NGS) technology (Roche 454), to generate large quantities of high-quality MLST data at low costs. The HiMLST protocol consists of two steps. In the first step MLST target genes are amplified by PCR in multi-well plates. During this PCR the amplicons of each bacterial isolate are provided with a unique DNA barcode, the multiplex identifier (MID). In the second step all amplicons are pooled and sequenced in a single NGS-run. The MLST profile of each individual isolate can be retrieved easily using its unique MID. With HiMLST we have profiled 575 isolates of Legionella pneumophila, Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus pneumoniae in mixed species HiMLST experiments. In conclusion, the introduction of HiMLST paves the way for a broad employment of the MLST as a high-quality and cost-effective method for typing microbial species.
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Registered trials
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