ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2012
Isoflurane binds and stabilizes a closed conformation of the leukocyte function-associated antigen-1.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 40 citations in OpenAlex.
- IQGAP1 Influences Neutrophil Maturation and Its Effector Functions.European journal of immunology · 2025Article
- Mechanism of isoflurane‑mediated breast cancer growthOncology letters · 2024Article
- Isoflurane attenuates sepsis-associated lung injury.Biochemical and biophysical research communications · 2022Article
- The immunomodulatory mechanism of dexmedetomidine.International immunopharmacology · 2021Review
- Natural killer cells in pancreatic cancer stroma.World journal of gastroenterology · 2021Review
- Anesthetics isoflurane and sevoflurane attenuate flagellin-mediated inflammation in the lung.Biochemical and biophysical research communications · 2021Article
- Risk factors for pediatric surgical site infection following neurosurgical procedures for hydrocephalus: a retrospective single-center cohort study.BMC anesthesiology · 2021Article
- Molecular Aspects of Volatile Anesthetic-Induced Organ Protection and Its Potential in Kidney Transplantation.International journal of molecular sciences · 2021Review
- Mechanistic consideration of the effect of perioperative volatile anesthetics on phagocytes.Clinical immunology (Orlando, Fla.) · 2021Review
- The Role of General Anesthetic Drug Selection in Cancer Outcome.BioMed research international · 2021Review
- The Role of Anesthetic Selection in Perioperative Bleeding.BioMed research international · 2021Review
- Surgical Site Infections and Perioperative Optimization of Host Immunity by Selection of Anesthetics.BioMed research international · 2021Review
- The effect of anesthetics on toll like receptor 9.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Volatile Anesthetic Sevoflurane Attenuates Toll-Like Receptor 1/2 Activation.Anesthesia and analgesia · 2020Article
- Article
- Volatile anesthetics isoflurane and sevoflurane directly target and attenuate Toll-like receptor 4 system.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- The Use of Volatile Anesthetics as Sedatives for Acute Respiratory Distress Syndrome.Translational perioperative and pain medicine · 2019Article
- Volatile anesthetics affect macrophage phagocytosis.PloS one · 2019Article
- Sites and Functional Consequence of Alkylphenol Anesthetic Binding to Kv1.2 Channels.Molecular neurobiology · 2018Article
- Volatile Anesthetic Isoflurane Attenuates Liver Injury in Experimental Polymicrobial Sepsis Model.Translational perioperative and pain medicine · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
We previously demonstrated that isoflurane targets lymphocyte function-associated antigen-1 (LFA-1), a critical adhesion molecule for leukocyte arrest. However, it remains to be determined how isoflurane interacts with the full ectodomain LFA-1 and modulates its conformation and function. Isoflurane binding sites on the full ectodomain LFA-1 were probed by photolabeling using photoactivatable isoflurane (azi-isoflurane). The adducted residues were determined by liquid chromatography/mass spectrometry analysis. Separately, docking simulations were performed to predict binding sites. Point mutations were introduced around isoflurane binding sites. The significance of isoflurane's effect was assessed in both intracellular adhesion molecule-1 (ICAM-1) binding assays and epitope mapping of activation-sensitive antibodies using flow cytometry. Two isoflurane binding sites were identified using photolabeling and were further validated by the docking simulation: one at the hydrophobic pocket in the ICAM-1 binding domain (the αI domain); the other at the βI domain. Mutagenesis of the α'1 helix showed that isoflurane binding sites at the βI domain were significantly important in modulating LFA-1 function and conformation. Epitope mapping using activation-sensitive antibodies suggested that isoflurane stabilized LFA-1 in the closed conformation. This study suggested that isoflurane binds to both the αI and βI domains allosteric to the ICAM-1 binding site, and that isoflurane binding stabilizes LFA-1 in the closed conformation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.