ArticleJournal of immunology (Baltimore, Md. : 1950)2012
A genetically encoded IL-1β bioluminescence resonance energy transfer sensor to monitor inflammasome activity.
Article in Journal of immunology (Baltimore, Md. : 1950), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- ASC oligomer favors caspase-1CARD domain recruitment after intracellular potassium efflux.The Journal of cell biology · 2023Article
- Measuring IL-1β Processing by Bioluminescence Sensors: Using a Bioluminescence Resonance Energy Transfer Biosensor.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Cellular Models and Assays to Study NLRP3 Inflammasome Biology.International journal of molecular sciences · 2020Review
- Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
- O-GlcNacylation Links TxNIP to Inflammasome Activation in Pancreatic β Cells.Frontiers in endocrinology · 2019Article
- In vivo imaging of inflammasome activation reveals a subcapsular macrophage burst response that mobilizes innate and adaptive immunity.Nature medicine · 2016Article
- Interleukin-10 modulates antigen presentation by dendritic cells through regulation of NLRP3 inflammasome assembly during Chlamydia infection.Infection and immunity · 2015Article
- Transgenic mouse model for imaging of interleukin-1β-related inflammation in vivo.Scientific reports · 2015Article
- Apoptosis-associated speck-like protein containing a CARD forms specks but does not activate caspase-1 in the absence of NLRP3 during macrophage swelling.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Bioluminescence: a versatile technique for imaging cellular and molecular features.MedChemComm · 2014Article
- Biosensors in clinical practice: focus on oncohematology.Sensors (Basel, Switzerland) · 2013Review
- BRET Biosensor Analysis of Receptor Tyrosine Kinase Functionality.Frontiers in endocrinology · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Inflammation is fundamental for protecting the organism against infection and injury. However, a failure to control immune response results in chronic inflammation and several associated disorders such as pain and loss of function. Initiation of inflammation is orchestrated by cytokines, among which IL-1β is particularly important. IL-1β is synthesized as an inactive protein that has to be processed by the inflammasome to generate the mature bioactive form. Conventional techniques cannot monitor IL-1β activation with high spatial and temporal resolution. In this study, we present a ratiometric biosensor that allows monitoring IL-1β processing in real time, with a temporal resolution of seconds and with a single-cell spatial resolution. Using this sensor, to our knowledge, we describe for the first time the kinetic of the inflammasome activity in living macrophages. With this new probe, we also demonstrated that the pro-IL-1β processing occurs all over the cytoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.