ArticleThe Journal of general virology2012
Permissive and restricted virus infection of murine embryonic stem cells.
Article in The Journal of general virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Pooled it
- Double stranded RNA sensing is silenced during early embryonic development.Nature communications · 2025Article
- Attenuated innate immunity in embryonic stem cells: mechanisms and therapeutic applications.Cell & bioscience · 2025Review
- Mouse Trophoblast Cells Have Attenuated Responses to TNF-α and IFN-γ and Can Avoid Synergic Cytotoxicity of the Two Cytokines.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Mouse Trophoblast Cells Can Provide IFN-Based Antiviral Protection to Embryonic Stem Cells via Paracrine Signaling.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- Dicer and PKR as Novel Regulators of Embryonic Stem Cell Fate and Antiviral Innate Immunity.Journal of immunology (Baltimore, Md. : 1950) · 2022Review
- Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells.Cell research · 2021Article
- Influenza a virus-triggered autophagy decreases the pluripotency of human-induced pluripotent stem cells.Cell death & disease · 2019Article
- The underdeveloped innate immunity in embryonic stem cells: The molecular basis and biological perspectives from early embryogenesis.American journal of reproductive immunology (New York, N.Y. : 1989) · 2019Article
- Characterization of Influenza A Virus Infection in Mouse Pulmonary Stem/Progenitor Cells.Frontiers in microbiology · 2019Article
- The Molecular Basis for the Lack of Inflammatory Responses in Mouse Embryonic Stem Cells and Their Differentiated Cells.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Utilization of different anti-viral mechanisms by mammalian embryonic stem cells and differentiated cells.Immunology and cell biology · 2017Review
- Derivation of chicken induced pluripotent stem cells tolerant to Newcastle disease virus-induced lysis through multiple rounds of infection.Virology journal · 2016Article
- Development of Antiviral Innate Immunity During In Vitro Differentiation of Mouse Embryonic Stem Cells.Stem cells and development · 2016Article
- Attenuated Innate Immunity in Embryonic Stem Cells and Its Implications in Developmental Biology and Regenerative Medicine.Stem cells (Dayton, Ohio) · 2015Review
- Conditional-ready mouse embryonic stem cell derived macrophages enable the study of essential genes in macrophage function.Scientific reports · 2015Article
- Enzyme regulation. IRBIT is a novel regulator of ribonucleotide reductase in higher eukaryotes.Science (New York, N.Y.) · 2014Article
- Antiviral responses in mouse embryonic stem cells: differential development of cellular mechanisms in type I interferon production and response.The Journal of biological chemistry · 2014Article
- Mouse embryonic stem cells are deficient in type I interferon expression in response to viral infections and double-stranded RNA.The Journal of biological chemistry · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
Recent RNA interference (RNAi) studies have identified many host proteins that modulate virus infection, but small interfering RNA 'off-target' effects and the use of transformed cell lines limit their conclusiveness. As murine embryonic stem (mES) cells can be genetically modified and resources exist where many and eventually all known mouse genes are insertionally inactivated, it was reasoned that mES cells would provide a useful alternative to RNAi screens. Beyond allowing investigation of host-pathogen interactions in vitro, mES cells have the potential to differentiate into other primary cell types, as well as being used to generate knockout mice for in vivo studies. However, mES cells are poorly characterized for virus infection. To investigate whether ES cells can be used to explore host-virus interactions, this study characterized the responses of mES cells following infection by herpes simplex virus type 1 (HSV-1) and influenza A virus. HSV-1 replicated lytically in mES cells, although mES cells were less permissive than most other cell types tested. Influenza virus was able to enter mES cells and express some viral proteins, but the replication cycle was incomplete and no infectious virus was produced. Knockdown of the host protein AHCYL1 in mES cells reduced HSV-1 replication, showing the potential for using mES cells to study host-virus interactions. Transcriptional profiling, however, indicated the lack of an efficient innate immune response in these cells. mES cells may thus be useful to identify host proteins that play a role in virus replication, but they are not suitable to determine factors that are involved in innate host defence.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.