ArticlePharmaceutical research2012
Penetration of fluorescein across the rabbit cornea from the endothelial surface.
Article in Pharmaceutical research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed, 22 citations in OpenAlex.
- Imaging-Based Drug Penetration Profiling in an Excised Sheep Cornea Model.Pharmaceutics · 2024Article
- Endothelial tissue remodeling induced by intraluminal pressure enhances paracellular solute transport.iScience · 2023Article
- Asymmetry in Drug Permeability through the Cornea.Pharmaceutics · 2021Article
- Computational modeling of drug transport across the in vitro cornea.Computers in biology and medicine · 2018Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo model the kinetics of penetration of fluorescein across the cornea from the endothelial surface.
methodsRabbit corneas mounted in vitro were exposed to fluorescein at their endothelial surface. Trans-corneal fluorescence were acquired periodically for 6 h using a custom-built confocal microfluorometer. The profiles were then employed to fit a kinetic model for calculation of permeability and diffusion coefficients across the cellular layers and stroma, respectively.
resultsAt the endothelium-stroma and stroma-epithelium interfaces, the fluorescence profile exhibited sudden jumps. In each case, the fluorescence was higher at the stroma, indicating reduced partitioning of the dye into the lipid-rich cellular layers. The stroma did not swell significantly until 180 min of perfusion. The fluorescence profiles reached a pseudo-steady state at ~6 h. A transport model, which included convective and diffusive fluxes into the stroma, showed a good fit to the trans-corneal profiles at different time points. The estimated permeability coefficients for the cellular layers were close to the values reported previously, but the diffusion coefficient of fluorescein in the stroma was found to be smaller than the values obtained previously using Ussing chambers.
conclusionsThe penetration of fluorescein could be modeled accurately by a combination of diffusion and convection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.