ArticleVirchows Archiv : an international journal of pathology2012
Systematic comparison of tissue fixation with alternative fixatives to conventional tissue fixation with buffered formalin in a xenograft-based model.
Article in Virchows Archiv : an international journal of pathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Spotlight on challenges and novel methods in highly multiplexed tissue imaging-based spatial proteomics.Journal of translational medicine · 2026Review
- Overlap of Formalin-Fixed Paraffin-Embedded and Fresh-Frozen Matched Tissues for Proteomics and Phosphoproteomics.ACS omega · 2025Article
- Coupling imaging mass cytometry with Alcian blue histochemical staining for a single-slide approach.Frontiers in immunology · 2024Article
- Preserving the Phosphoproteome of Clinical Biopsies Using a Quick-Freeze Collection Device.Biopreservation and biobanking · 2022Article
- Article
- Formalin fixation for optimal concordance of programmed death-ligand 1 immunostaining between cytologic and histologic specimens from patients with non-small cell lung cancer.Cancer cytopathology · 2021Article
- Article
- A Buffered Alcohol-Based Fixative for Histomorphologic and Molecular Applications.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2016Article
- Article
- Immunohistochemical demonstration of specific antigens in the human brain fixed in zinc-ethanol-formaldehyde.European journal of histochemistry : EJH · 2015Article
- Tissue fixation and the effect of molecular fixatives on downstream staining procedures.Methods (San Diego, Calif.) · 2014Review
- Proper paraffin slide storage is crucial for translational research projects involving immunohistochemistry stains.Clinical and translational medicine · 2014Article
- A review of room temperature storage of biospecimen tissue and nucleic acids for anatomic pathology laboratories and biorepositories.Clinical biochemistry · 2014Review
- Reply to the comment of Drs Goldman and Vollmer.Virchows Archiv : an international journal of pathology · 2013Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In our study we systematically compared the alternative fixatives acidified formal alcohol (AFA), PAXgene®, HOPE®, and combinations of AFA or formalin with ultrasound treatment to standard (buffered) formalin fixation. We examined general morphology and detectability of protein structures by immunohistochemistry of the membrane receptors epidermal growth factor receptor (EGFR), insulin-like growth factor 1 receptor (IGF-1R), and phosphorylated human epidermal growth factor receptor 2 (phospho-HER2). In order to allow for stringent comparability of different fixation techniques, we used matched mouse xenograft tumor samples from three different human cancer cell lines (colon, ovarian, and non-small cell lung cancer), either fixed conventionally with formalin or an alternative fixative. Tissue morphology after fixation with AFA and PAXgene® was comparable to formalin-fixed paraffin-embedded tissue (FFPET) morphology. Ultrasound fixations resulted in slightly inferior morphology and HOPE® fixation preserved morphology only poorly compared to FFPET in this system. None of the tested alternative fixatives enabled immunohistochemical detectability of all three targets in the same manner as FFPET. Pronounced staining was possible for EGFR and IGF-1R with all alternative fixatives but HOPE®, and phospho-HER2 staining was only noteworthy with formalin-ultrasound-fixed tissue. Therefore, the use of alternative fixatives comes with the need for careful validation of obtained IHC results individually for each target.
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