ArticleDevelopmental cell2012
Separase biosensor reveals that cohesin cleavage timing depends on phosphatase PP2A(Cdc55) regulation.
Article in Developmental cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 47 citations in OpenAlex.
- Chromosome segregation synchrony in S. pombe is noise limited and arises without positive feedback.The Journal of cell biology · 2026Article
- Chromosome segregation synchrony inbioRxiv : the preprint server for biology · 2026Article
- A coordinated kinase and phosphatase network regulates Stu2 recruitment to yeast kinetochores.The Journal of cell biology · 2025Article
- Securin regulates the spatiotemporal dynamics of separase.The Journal of cell biology · 2025Article
- Meiotic cells escape prolonged spindle checkpoint activity through kinetochore silencing and slippage.PLoS genetics · 2023Article
- The Polo kinase Cdc5 is regulated at multiple levels in the adaptation response to telomere dysfunction.Genetics · 2023Article
- Meiotic Cells Escape Prolonged Spindle Checkpoint Activity Through Premature Silencing and Slippage.bioRxiv : the preprint server for biology · 2023Article
- SUMOylation stabilizes sister kinetochore biorientation to allow timely anaphase.The Journal of cell biology · 2021Article
- Structure and Function of the Separase-Securin Complex.Sub-cellular biochemistry · 2021Review
- PP2A Functions during Mitosis and Cytokinesis in Yeasts.International journal of molecular sciences · 2019Review
- The Opposing Functions of Protein Kinases and Phosphatases in Chromosome Bipolar Attachment.International journal of molecular sciences · 2019Review
- Reductional Meiosis I Chromosome Segregation Is Established by Coordination of Key Meiotic Kinases.Developmental cell · 2019Article
- Ser/Thr protein phosphatases in fungi: structure, regulation and function.Microbial cell (Graz, Austria) · 2019Review
- Review
- The DNA Damage Checkpoint and the Spindle Position Checkpoint Maintain Meiotic Commitment in Saccharomyces cerevisiae.Current biology : CB · 2019Article
- Spo13 prevents premature cohesin cleavage during meiosis.Wellcome open research · 2019Article
- SILAC-based phosphoproteomics reveals new PP2A-Cdc55-regulated processes in budding yeast.GigaScience · 2018Article
- Cdk1 phosphorylation of Esp1/Separase functions with PP2A and Slk19 to regulate pericentric Cohesin and anaphase onset.PLoS genetics · 2018Article
- Functions and regulation of the Polo-like kinase Cdc5 in the absence and presence of DNA damage.Current genetics · 2018Review
- Firing of Replication Origins Frees Dbf4-Cdc7 to Target Eco1 for Destruction.Current biology : CB · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In anaphase, sister chromatids separate abruptly and are then segregated by the mitotic spindle. The protease separase triggers sister separation by cleaving the Scc1/Mcd1 subunit of the cohesin ring that holds sisters together. Polo-kinase phosphorylation of Scc1 promotes its cleavage, but the underlying regulatory circuits are unclear. We developed a separase biosensor in Saccharomyces cerevisiae that provides a quantitative indicator of cohesin cleavage in single cells. Separase is abruptly activated and cleaves most cohesin within 1 min, after which anaphase begins. Cohesin near centromeres and telomeres is cleaved at the same rate and time. Protein phosphatase PP2A(Cdc55) inhibits cohesin cleavage by counteracting polo-kinase phosphorylation of Scc1. In early anaphase, the previously described separase inhibition of PP2A(Cdc55) promotes cohesin cleavage. Thus, separase acts directly on Scc1 and also indirectly, through inhibition of PP2A(Cdc55), to stimulate cohesin cleavage, providing a feedforward loop that may contribute to a robust and timely anaphase.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.