ArticleCell reports2012
Structure of a peptidoglycan amidase effector targeted to Gram-negative bacteria by the type VI secretion system.
Article in Cell reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 108 citations in OpenAlex.
- Type VI-secretion system-mediated competition and virulence in clinical and environmentalMicrobiology spectrum · 2026Article
- Systematic identification of Salmonella T6SS effectors uncovers diverse new families and lipid-targeting activities.PLoS biology · 2026Article
- Two cysteines control Tse1 secretion by H1-T6SS in Pseudomonas aeruginosa.Protein science : a publication of the Protein Society · 2025Article
- Rapidly evolving orphan immunity genes protect human gut bacteria from intoxication by the type VI secretion system.bioRxiv : the preprint server for biology · 2025Article
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- Delivery determinants of anmBio · 2025Article
- Two-step localization driven by peptidoglycan hydrolase in interbacterial predation.The ISME journal · 2025Article
- A coordinated attack by a bacterial secretion system and a small molecule drives prey specificity.Communications biology · 2024Article
- The nucleoid protein HU positively regulates the expression of type VI secretion systems inmSphere · 2024Article
- NlpC/P60 peptidoglycan hydrolases of Trichomonas vaginalis have complementary activities that empower the protozoan to control host-protective lactobacilli.PLoS pathogens · 2023Article
- Qualitative and Quantitative Methods to Measure Antibacterial Activity Resulting from Bacterial Competition.Bio-protocol · 2023Article
- Lipopolysaccharide transport regulates bacterial sensitivity to a cell wall-degrading intermicrobial toxin.PLoS pathogens · 2023Article
- Lipopolysaccharide integrity primes bacterial sensitivity to a cell wall-degrading intermicrobial toxin.bioRxiv : the preprint server for biology · 2023Article
- Bacterial type VI secretion system (T6SS): an evolved molecular weapon with diverse functionality.Biotechnology letters · 2023Review
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- Killing of Gram-negative and Gram-positive bacteria by a bifunctional cell wall-targeting T6SS effector.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
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- Horizontally acquired antibacterial genes associated with adaptive radiation of ladybird beetles.BMC biology · 2021Article
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2 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The target range of a bacterial secretion system can be defined by effector substrate specificity or by the efficacy of effector delivery. Here, we report the crystal structure of Tse1, a type VI secretion (T6S) bacteriolytic amidase effector from Pseudomonas aeruginosa. Consistent with its role as a toxin, Tse1 has a more accessible active site than related housekeeping enzymes. The activity of Tse1 against isolated peptidoglycan shows its capacity to act broadly against Gram-negative bacteria and even certain Gram-positive species. Studies with intact cells indicate that Gram-positive bacteria can remain vulnerable to Tse1 despite cell wall modifications. However, interbacterial competition studies demonstrate that Tse1-dependent lysis is restricted to Gram-negative targets. We propose that the previously observed specificity for T6S against Gram-negative bacteria is a consequence of high local effector concentration achieved by T6S-dependent targeting to its site of action rather than inherent effector substrate specificity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.