ArticleJournal of bioenergetics and biomembranes2012
Monitoring of real changes of plasma membrane potential by diS-C(3)(3) fluorescence in yeast cell suspensions.
Article in Journal of bioenergetics and biomembranes, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed.
- From the Metabolic Effects and Mechanism of Monovalent Cation Transport to the Actual Measurement of the Plasma Membrane Potential in Yeast.Journal of fungi (Basel, Switzerland) · 2025Review
- The Use of Thioflavin T for the Estimation and Measurement of the Plasma Membrane Electric Potential Difference in Different Yeast Strains.Journal of fungi (Basel, Switzerland) · 2023Article
- Bioelectronics communication: encoding yeast regulatory responses using nanostructured gallium nitride thin films.Nanoscale · 2018Article
- Acridine yellow. A novel use to estimate and measure the plasma membrane potential in Saccharomyces cerevisiae.Journal of bioenergetics and biomembranes · 2017Article
- Computational models for monitoring the trans-membrane potential with fluorescent probes: the DiSCEuropean biophysics journal : EBJ · 2016Article
- Estimation of Candida albicans ABC Transporter Behavior in Real-Time via Fluorescence.Frontiers in microbiology · 2015Article
- Quantitative description of ion transport via plasma membrane of yeast and small cells.Frontiers in plant science · 2015Review
- Complementary Methods of Processing diS-C3(3) Fluorescence Spectra Used for Monitoring the Plasma Membrane Potential of Yeast: Their Pros and Cons.Journal of fluorescence · 2014Article
- Early changes in membrane potential of Saccharomyces cerevisiae induced by varying extracellular K(+), Na (+) or H (+) concentrations.Journal of bioenergetics and biomembranes · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fluorescent dye 3,3'-dipropylthiadicarbocyanine, diS-C(3)(3), is a suitable probe to monitor real changes of plasma membrane potential in yeast cells which are too small for direct membrane potential measurements with microelectrodes. A method presented in this paper makes it possible to convert changes of equilibrium diS-C(3)(3) fluorescence spectra, measured in yeast cell suspensions under certain defined conditions, into underlying membrane potential differences, scaled in the units of millivolts. Spectral analysis of synchronously scanned diS-C(3)(3) fluorescence allows to assess the amount of dye accumulated in cells without otherwise necessary sample taking and following separation of cells from the medium. Moreover, membrane potential changes can be quantified without demanding calibration protocols. The applicability of this approach was demonstrated on the depolarization of Rhodotorula glutinis yeast cells upon acidification of cell suspensions and/or by increasing extracellular K(+) concentration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.