ArticleMolecular biology of the cell2012
Cell cycle-regulated cortical dynein/dynactin promotes symmetric cell division by differential pole motion in anaphase.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 71 citations in OpenAlex.
- Article
- Torques within and outside the human spindle balance twist at anaphase.The Journal of cell biology · 2024Article
- Broad misappropriation of developmental splicing profile by cancer in multiple organs.Nature communications · 2022Article
- Membrane compartmentalization of Ect2/Cyk4/Mklp1 and NuMA/dynein regulates cleavage furrow formation.The Journal of cell biology · 2022Article
- Developing Genetic Engineering Techniques for Control of Seed Size and Yield.International journal of molecular sciences · 2022Review
- Dynactin 2 acts as an oncogene in hepatocellular carcinoma through promoting cell cycle progression.Liver research (Beijing, China) · 2022Article
- An RNAi screen for genes that affect nuclear morphology in Caenorhabditis elegans reveals the involvement of unexpected processes.G3 (Bethesda, Md.) · 2021Article
- The Nuclear Mitotic Apparatus (NuMA) Protein: A Key Player for Nuclear Formation, Spindle Assembly, and Spindle Positioning.Frontiers in cell and developmental biology · 2021Review
- PP2A--B55γ counteracts Cdk1 and regulates proper spindle orientation through the cortical dynein adaptor NuMA.Journal of cell science · 2020Article
- Unconventional Roles of Cytoskeletal Mitotic Machinery in Neurodevelopment.Trends in cell biology · 2019Review
- Mechanisms of Spindle Positioning: Lessons from Worms and Mammalian Cells.Biomolecules · 2019Review
- The polarity-induced force imbalance inMolecular biology of the cell · 2018Article
- Plk1 regulates spindle orientation by phosphorylating NuMA in human cells.Life science alliance · 2018Article
- Num1 versus NuMA: insights from two functionally homologous proteins.Biophysical reviews · 2018Review
- Spindle rotation in human cells is reliant on a MARK2-mediated equatorial spindle-centering mechanism.The Journal of cell biology · 2018Article
- PKCε Controls Mitotic Progression by Regulating Centrosome Migration and Mitotic Spindle Assembly.Molecular cancer research : MCR · 2018Article
- Kif2 localizes to a subdomain of cortical endoplasmic reticulum that drives asymmetric spindle position.Nature communications · 2017Article
- Microtubule cross-linking activity of She1 ensures spindle stability for spindle positioning.The Journal of cell biology · 2017Article
- Increased lateral microtubule contact at the cell cortex is sufficient to drive mammalian spindle elongation.Molecular biology of the cell · 2017Article
- Automated mitotic spindle tracking suggests a link between spindle dynamics, spindle orientation, and anaphase onset in epithelial cells.Molecular biology of the cell · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In cultured mammalian cells, how dynein/dynactin contributes to spindle positioning is poorly understood. To assess the role of cortical dynein/dynactin in this process, we generated mammalian cell lines expressing localization and affinity purification (LAP)-tagged dynein/dynactin subunits from bacterial artificial chromosomes and observed asymmetric cortical localization of dynein and dynactin during mitosis. In cells with asymmetrically positioned spindles, dynein and dynactin were both enriched at the cortex distal to the spindle. NuMA, an upstream targeting factor, localized asymmetrically along the cell cortex in a manner similar to dynein and dynactin. During spindle motion toward the distal cortex, dynein and dynactin were locally diminished and subsequently enriched at the new distal cortex. At anaphase onset, we observed a transient increase in cortical dynein, followed by a reduction in telophase. Spindle motion frequently resulted in cells entering anaphase with an asymmetrically positioned spindle. These cells gave rise to symmetric daughter cells by dynein-dependent differential spindle pole motion in anaphase. Our results demonstrate that cortical dynein and dynactin dynamically associate with the cell cortex in a cell cycle-regulated manner and are required to correct spindle mispositioning in LLC-Pk1 epithelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.