Evidence map›Paper›PMID 22809504›Full record

ArticleBiochemical and biophysical research communications2012

Coxsackievirus and adenovirus receptor (CAR) mediates trafficking of acid sensing ion channel 3 (ASIC3) via PSD-95.

Katherine J D A Excoffon, Abimbola O Kolawole, Nobuyoshi Kusama, Nicholas D Gansemer, Priyanka Sharma, Alesia M Hruska-Hageman, Elena Petroff, Christopher J Benson

Abstract read
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In one paragraph

Article in Biochemical and biophysical research communications, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Katherine J D A ExcoffonDepartment of Biological Sciences, Wright State University, Dayton, OH 45435, USA. katherine.excoffon@wright.edu
Abimbola O Kolawole
Nobuyoshi Kusama
Nicholas D Gansemer
Priyanka Sharma
Alesia M Hruska-Hageman
Elena Petroff
Christopher J Benson

Funding

Acid-Sensing Ion Channels in Cardiac IschemiaR01HL076419 · NHLBI · UNIVERSITY OF IOWA · PI BENSON, CHRISTOPHER J · 2005 to 2009
$2.0M
Isoform-specific Regulation of the Coxsackie and Adenovirus Receptor in Polarized EpitheliaR15AI090625 · NIAID · WRIGHT STATE UNIVERSITY · PI EXCOFFON, KATHERINE JULIE · 2010 to 2015
$881k
Interaction of ASIC and BK channels and its role in glial proliferationR15NS070260 · NINDS · MONTCLAIR STATE UNIVERSITY · PI PETROFF, ELENA · 2011 to 2011
$321k
NHLBI NIH HHS HL076419NHLBI NIH HHS R01 HL076419NIAID NIH HHS R15 AI090625NIAID NIH HHS R15AI090625NINDS NIH HHS R15 NS070260NINDS NIH HHS R15NS070260
6 · The paper itself

Abstract

We have previously shown that the Coxsackievirus and adenovirus receptor (CAR) can interact with post-synaptic density 95 (PSD-95) and localize PSD-95 to cell-cell junctions. We have also shown that activity of the acid sensing ion channel (ASIC3), a H(+)-gated cation channel that plays a role in mechanosensation and pain signaling, is negatively modulated by PSD-95 through a PDZ-based interaction. We asked whether CAR and ASIC3 simultaneously interact with PSD-95, and if so, whether co-expression of these proteins alters their cellular distribution and localization. Results indicate that CAR and ASIC3 co-immunoprecipitate only when co-expressed with PSD-95. CAR also brings both PSD-95 and ASIC3 to the junctions of heterologous cells. Moreover, CAR rescues PSD-95-mediated inhibition of ASIC3 currents. These data suggest that, in addition to activity as a viral receptor and adhesion molecule, CAR can play a role in trafficking proteins, including ion channels, in a PDZ-based scaffolding complex.

Indexed as

Acid Sensing Ion ChannelsAnimalsChlorocebus aethiopsCOS CellsCoxsackie and Adenovirus Receptor-Like Membrane ProteinDisks Large Homolog 4 ProteinHumansImmunoprecipitationIntracellular Signaling Peptides and ProteinsMembrane ProteinsPDZ DomainsProtein TransportAcid Sensing Ion ChannelsASIC3 protein, humanCLMP protein, humanCoxsackie and Adenovirus Receptor-Like Membrane ProteinDisks Large Homolog 4 ProteinDLG4 protein, humanIntracellular Signaling Peptides and ProteinsMembrane Proteins

Identifiers

PMID22809504
PMCPMC3423544

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.