ArticleBiotechnology for biofuels2012
Improved ethanol production by a xylose-fermenting recombinant yeast strain constructed through a modified genome shuffling method.
Article in Biotechnology for biofuels, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Intergeneric chromosomal transfer in yeast results in improved phenotypes and widespread transcriptional responses.Nature communications · 2026Article
- Recent progress in engineering yeast producers of cellulosic ethanol.FEMS yeast research · 2025Review
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- Modern yeast development: finding the balance between tradition and innovation in contemporary winemaking.FEMS yeast research · 2023Review
- Whole-Genome Transformation of Yeast Promotes Rare Host Mutations with a Single Causative SNP Enhancing Acetic Acid Tolerance.Molecular and cellular biology · 2022Article
- Article
- A plasmid-freeFrontiers in bioengineering and biotechnology · 2022Article
- A novel AST2 mutation generated upon whole-genome transformation of Saccharomyces cerevisiae confers high tolerance to 5-Hydroxymethylfurfural (HMF) and other inhibitors.PLoS genetics · 2021Article
- Mechanisms underlying lactic acid tolerance and its influence on lactic acid production inMicrobial cell (Graz, Austria) · 2021Review
- Article
- Repeated batches as a strategy for high 2G ethanol production from undetoxified hemicellulose hydrolysate using immobilized cells of recombinantBiotechnology for biofuels · 2020Article
- Development and characterization of acidic-pH-tolerant mutants ofBiotechnology for biofuels · 2020Article
- Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha).Journal of industrial microbiology & biotechnology · 2020Review
- Integration of a multi-step heterologous pathway in Saccharomyces cerevisiae for the production of abscisic acid.Microbial cell factories · 2019Article
- Genome shuffling enhances stress tolerance ofBiotechnology for biofuels · 2019Article
- Improvement ofFood science and biotechnology · 2018Article
- Genome and metabolic engineering in non-conventional yeasts: Current advances and applications.Synthetic and systems biotechnology · 2017Review
- Engineering tolerance to industrially relevant stress factors in yeast cell factories.FEMS yeast research · 2017Review
- Overexpression of smORF YNR034W-A/EGO4 in Saccharomyces cerevisiae increases the fermentative efficiency of Agave tequilana Weber must.Journal of industrial microbiology & biotechnology · 2017Article
- Expression, Docking, and Molecular Dynamics of Endo-BioMed research international · 2017Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundXylose is the second most abundant carbohydrate in the lignocellulosic biomass hydrolysate. The fermentation of xylose is essential for the bioconversion of lignocelluloses to fuels and chemicals. However the wild-type strains of Saccharomyces cerevisiae are unable to utilize xylose. Many efforts have been made to construct recombinant yeast strains to enhance xylose fermentation over the past few decades. Xylose fermentation remains challenging due to the complexity of lignocellulosic biomass hydrolysate. In this study, a modified genome shuffling method was developed to improve xylose fermentation by S. cerevisiae. Recombinant yeast strains were constructed by recursive DNA shuffling with the recombination of entire genome of P. stipitis with that of S. cerevisiae.
resultsAfter two rounds of genome shuffling and screening, one potential recombinant yeast strain ScF2 was obtained. It was able to utilize high concentration of xylose (100 g/L to 250 g/L xylose) and produced ethanol. The recombinant yeast ScF2 produced ethanol more rapidly than the naturally occurring xylose-fermenting yeast, P. stipitis, with improved ethanol titre and much more enhanced xylose tolerance.
conclusionThe modified genome shuffling method developed in this study was more effective and easier to operate than the traditional protoplast-fusion-based method. Recombinant yeast strain ScF2 obtained in this study was a promising candidate for industrial cellulosic ethanol production. In order to further enhance its xylose fermentation performance, ScF2 needs to be additionally improved by metabolic engineering and directed evolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.