ArticlePloS one2012
Inactivation of Candida biofilms by non-thermal plasma and its enhancement for fungistatic effect of antifungal drugs.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Cellular responses to prolonged non-thermal plasma exposure in Schizosaccharomyces pombe.Applied microbiology and biotechnology · 2026Article
- Antimicrobial efficacy and cytotoxic assessment of plasma-activated water generated by a dielectric barrier discharge microbubble system.Frontiers in microbiology · 2026Article
- Understanding Heterogeneity and Tolerance of Dual Candida albicans-Staphylococcus aureus Biofilms to Cold Atmospheric Plasma and Antimicrobial Combinations.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2025Article
- Comparing the Effect of Photodynamic Therapy With 660 and 450 nm Lasers and Cold Atmospheric Plasma onJournal of lasers in medical sciences · 2025Article
- Cold atmospheric plasma improves antifungal responsiveness of Aspergillus flavus and Fusarium keratoplasticum conidia and mycelia.PloS one · 2025Article
- Cold atmospheric plasma inactivatesJournal of medical microbiology · 2024Article
- Article
- Could Helium Plasma Treatment be a Novel Approach to Prevent the Biofilm Formation of Candida albicans?Mycopathologia · 2023Article
- Application of Non-Thermal Plasma to Fungal Resources.Journal of fungi (Basel, Switzerland) · 2022Review
- Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals onMolecules (Basel, Switzerland) · 2021Article
- Inactivation and sensitization of Pseudomonas aeruginosa by microplasma jet array for treating otitis media.NPJ biofilms and microbiomes · 2021Article
- The Antimicrobial Effect of Cold Atmospheric Plasma against Dental Pathogens-A Systematic Review of In-Vitro Studies.Antibiotics (Basel, Switzerland) · 2021Review
- Nonthermal plasma as part of a novel strategy for vaccination.Plasma processes and polymers (Print) · 2020Review
- Use of non-thermal plasma pre-treatment to enhance antibiotic action against mature Pseudomonas aeruginosa biofilms.World journal of microbiology & biotechnology · 2020Article
- Antimicrobial effects of microwave-induced plasma torch (MiniMIP) treatment on Candida albicans biofilms.Microbial biotechnology · 2019Article
- Medically important biofilms and non-thermal plasma.World journal of microbiology & biotechnology · 2018Review
- Disintegration of simulated drinking water biofilms with arrays of microchannel plasma jets.NPJ biofilms and microbiomes · 2018Article
- Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study.PloS one · 2018Article
- Antimicrobial strategies centered around reactive oxygen species--bactericidal antibiotics, photodynamic therapy, and beyond.FEMS microbiology reviews · 2013Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the antifungal effect of non-thermal plasma, as well as its combination with common antifungal drugs, against Candida biofilms. A direct current atmospheric pressure He/O(2) (2%) plasma microjet (PMJ) was used to treat Candida biofilms in a 96-well plate. Inactivation efficacies of the biofilms were evaluated by XTT assay and counting colony forming units (CFUs). Morphological properties of the biofilms were evaluated by Scanning Electron Microscope (SEM). The sessile minimal inhibitory concentrations (SMICs) of fluconazole, amphotericin B, and caspofungin for the biofilms were also tested. Electron Spin Resonance (ESR) spectroscopy was used to detect the reactive oxygen species (ROS) generated directly and indirectly by PMJ. The Candida biofilms were completely inactivated after 1 min PMJ treatment, where severely deformed fungal elements were observed in SEM images. The SMICs of the tested antifungal drugs for the plasma-treated biofilms were decreased by 2-6 folds of dilution, compared to those of the untreated controls. ROS such as hydroxyl radical ((•)OH), superoxide anion radical ((•)O(2) (-)) and singlet molecular oxygen ((1)O(2)) were detected by ESR. We hence conclude that He/O(2) (2%) plasma alone, as well as in combination with common antifungal drugs, is able to inactivate Candida biofilms rapidly. The generation of ROS is believed to be one of the underlying mechanisms for the fungicidal activity of plasma.
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