ArticleIn vitro cellular & developmental biology. Animal2012
Proteomic analysis of the nuclear phosphorylated proteins in dairy cow mammary epithelial cells treated with estrogen.
Article in In vitro cellular & developmental biology. Animal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- Review
- Exploration of Proteomics Analysis of Crop Milk in Pigeons (ACS omega · 2021Article
- Effects of a High-Grain Diet With a Buffering Agent on Milk Protein Synthesis in Lactating Goats.Frontiers in veterinary science · 2021Article
- DEAD-box helicase 6 (DDX6) is a new negative regulator for milk synthesis and proliferation of bovine mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2018Article
- Regulation of peroxisome proliferator-activated receptor gamma on milk fat synthesis in dairy cow mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2016Article
- Up-regulation of integrin α6β4 expression by mitogens involved in dairy cow mammary development.In vitro cellular & developmental biology. Animal · 2015Article
- Function of SREBP1 in the milk fat synthesis of dairy cow mammary epithelial cells.International journal of molecular sciences · 2014Article
- Leucyl-tRNA synthetase regulates lactation and cell proliferation via mTOR signaling in dairy cow mammary epithelial cells.International journal of molecular sciences · 2014Article
- SOCS3-mediated blockade reveals major contribution of JAK2/STAT5 signaling pathway to lactation and proliferation of dairy cow mammary epithelial cells in vitro.Molecules (Basel, Switzerland) · 2013Article
- Proteomic and functional analyses reveal MAPK1 regulates milk protein synthesis.Molecules (Basel, Switzerland) · 2012Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Estrogen regulates a variety of physiological processes, including mammary gland growth, morphogenesis of the mammary gland, proliferation and differentiation, and elevating the expression of milk proteins. Many nuclear phosphorylated proteins such as pStat5 and mTOR regulate milk protein synthesis. But the detail of milk protein synthesis controlled at the transcript level and posttranslational level is not well-known. To contribute to the understanding of the molecular mechanism underlying estrogen action on the dairy cow mammary epithelial cells (DCMECs), nuclear phosphorylated proteins regulated by estrogen in DCMECs were identified. Two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization/time of flight mass spectrometry were used to identify the changes of nuclear phosphorylated proteins in DCMECs treated with estrogen. Seven proteins were identified differentially up-expressed in DCMECs after 24-h estrogen exposure: including glycyl-tRNA synthetase, previously reported in milk protein synthesis of DCMECs, belonging to the class-II aminoacyl-tRNA synthetase family; proteins involved in other cellular functions, such as translation initiation factors, GTP-binding nuclear proteins, heat-shock proteins, and proteins belonging to ubiquitin-proteasome system. This screening reveals that estrogen influences the levels of nuclear phosphorylated proteins of DCMECs which opens new avenue for the study of the molecular mechanism linking to milk synthesis.
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