ArticleBlood2012
MKL1 and MKL2 play redundant and crucial roles in megakaryocyte maturation and platelet formation.
Article in Blood, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 60 citations in OpenAlex.
- RBM15 in leukemogenesis: an emerging regulator at the interface of RNA biology and hematopoiesis.Trends in cancer · 2026Review
- EPC1/2 regulate hematopoietic stem and progenitor cell proliferation by modulating H3 acetylation and DLST.iScience · 2024Article
- Structure-function analysis of the role of megakaryoblastic leukemia 1 in megakaryocyte polyploidization.Haematologica · 2022Article
- First Successful Allogeneic Hematopoietic Stem Cell Transplantation for MKL1 Deficiency.Journal of clinical immunology · 2022Article
- Relieving DYRK1A repression of MKL1 confers an adult-like phenotype to human infantile megakaryocytes.The Journal of clinical investigation · 2022Article
- Differential regulation of cranial and cardiac neural crest by serum response factor and its cofactors.eLife · 2022Article
- Serum response factor-cofactor interactions and their implications in disease.The FEBS journal · 2021Review
- Reprogramming progressive cells display low CAG promoter activity.Stem cells (Dayton, Ohio) · 2021Article
- MKL-1 is a coactivator for STAT5b, the regulator of Treg cell development and function.Cell communication and signaling : CCS · 2020Article
- An intronic deletion in megakaryoblastic leukemia 1 is associated with hyperproliferation of B cells in triplets with Hodgkin lymphoma.Haematologica · 2020Article
- MRTFB suppresses colorectal cancer development through regulating SPDL1 and MCAM.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Autoreactive B cells in SLE, villains or innocent bystanders?Immunological reviews · 2019Review
- Low iron promotes megakaryocytic commitment of megakaryocytic-erythroid progenitors in humans and mice.Blood · 2019Article
- Epithelial (E)-Cadherin is a Novel Mediator of Platelet Aggregation and Clot Stability.Thrombosis and haemostasis · 2019Article
- Therapeutic Targeting of Fibrotic Epithelial-Mesenchymal Transition-An Outstanding Challenge.Frontiers in pharmacology · 2019Review
- MRTFA augments megakaryocyte maturation by enhancing the SRF regulatory axis.Blood advances · 2018Article
- Megakaryocyte ontogeny: Clinical and molecular significance.Experimental hematology · 2018Review
- Serum Response Factor Is Essential for Maintenance of Podocyte Structure and Function.Journal of the American Society of Nephrology : JASN · 2018Article
- The myocardin-related transcription factor MKL co-regulates the cellular levels of two profilin isoforms.The Journal of biological chemistry · 2017Article
- Neonatal expression of RNA-binding protein IGF2BP3 regulates the human fetal-adult megakaryocyte transition.The Journal of clinical investigation · 2017Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Serum response factor and its transcriptional cofactor MKL1 are critical for megakaryocyte maturation and platelet formation. We show that MKL2, a homologue of MKL1, is expressed in megakaryocytes and plays a role in megakaryocyte maturation. Using a megakaryocyte-specific Mkl2 knockout (KO) mouse on the conventional Mkl1 KO background to produce double KO (DKO) megakaryocytes and platelets, a critical role for MKL2 is revealed. The decrease in megakaryocyte ploidy and platelet counts of DKO mice is more severe than in Mkl1 KO mice. Platelet dysfunction in DKO mice is revealed by prolonged bleeding times and ineffective platelet activation in vitro in response to adenosine 5'-diphosphate. Electron microscopy and immunofluorescence of DKO megakaryocytes and platelets indicate abnormal cytoskeletal and membrane organization with decreased granule complexity. Surprisingly, the DKO mice have a more extreme thrombocytopenia than mice lacking serum response factor (SRF) expression in the megakaryocyte compartment. Comparison of gene expression reveals approximately 4400 genes whose expression is differentially affected in DKO compared with megakaryocytes deficient in SRF, strongly suggesting that MKL1 and MKL2 have both SRF-dependent and SRF-independent activity in megakaryocytopoiesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.