ArticleWorld journal of microbiology & biotechnology2012
Selection of available suicide vectors for gene mutagenesis using chiA (a chitinase encoding gene) as a new reporter and primary functional analysis of chiA in Lysobacter enzymogenes strain OH11.
Article in World journal of microbiology & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
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- Quorum quenching by a type IVA secretion system effector.The ISME journal · 2023Article
- Impact of Two Phosphorus Fertilizer Formulations on Wheat Physiology, Rhizosphere, and Rhizoplane Microbiota.International journal of molecular sciences · 2023Article
- The Wsp chemosensory system modulates c-di-GMP-dependent biofilm formation by integrating DSF quorum sensing through the WspR-RpfG complex in Lysobacter.NPJ biofilms and microbiomes · 2022Article
- The First Homologous Expression System for the β-Lytic Protease ofInternational journal of molecular sciences · 2022Article
- Lysobacter arenosi sp. nov. and Lysobacter solisilvae sp. nov. isolated from soil.Journal of microbiology (Seoul, Korea) · 2021Article
- Coordinated control of the type IV pili and c-di-GMP-dependent antifungal antibiotic production in Lysobacter by the response regulator PilR.Molecular plant pathology · 2021Article
- Deletion ofFrontiers in microbiology · 2021Article
- Clp is a "busy" transcription factor in the bacterial warrior,Computational and structural biotechnology journal · 2021Review
- Knockout of Diguanylate Cyclase Genes in Lysobacter enzymogenes to Improve Production of Antifungal Factor and Increase Its Application in Seed Coating.Current microbiology · 2020Article
- A YajQ-LysR-like, cyclic di-GMP-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, Lysobacter enzymogenes.Molecular plant pathology · 2020Article
- Transition of the Bacterial Community and Culturable Chitinolytic Bacteria in Chitin-treated Upland Soil: From Streptomyces to Methionine-auxotrophic Lysobacter and Other Genera.Microbes and environments · 2020Article
- Two forms of phosphomannomutase in gammaproteobacteria: The overlooked membrane-bound form of AlgC is required for twitching motility of Lysobacter enzymogenes.Environmental microbiology · 2019Article
- Signaling specificity in the c-di-GMP-dependent network regulating antibiotic synthesis in Lysobacter.Nucleic acids research · 2018Article
- Heat-Stable Antifungal Factor (HSAF) Biosynthesis in Lysobacter enzymogenes Is Controlled by the Interplay of Two Transcription Factors and a Diffusible Molecule.Applied and environmental microbiology · 2018Article
- Spermidine-Regulated Biosynthesis of Heat-Stable Antifungal Factor (HSAF) inFrontiers in microbiology · 2018Article
- Lsp family proteins regulate antibiotic biosynthesis in Lysobacter enzymogenes OH11.AMB Express · 2017Article
- Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus.Marine drugs · 2017Article
- ChpA Controls Twitching Motility and Broadly Affects Gene Expression in the Biological Control Agent Lysobacter enzymogenes.Current microbiology · 2017Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
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Abstract
Here, three different suicide vectors were evaluated for the possibility of performing gene mutagenesis in strain OH11 using the chiA gene (accession number: DQ888611) as a new reporter. Suicide vector pEX18GM was selected, and it was successfully applied for disruption and in-frame deletions in the chiA gene in strain OH11, which was confirmed by PCR amplification and Southern hybridization. The chiA-deletion mutant OH11-3 did not have the ability to produce chitinase on chitine selection medium. Interestingly, the chiA-deletion mutants displayed wild-type antimicrobial activity against Saccharomyces cerevisiae, Magnaporthe grisea, Phytophthora capsici, Rhizoctonia solani, Sclerotinia sclerotiorum and Pythium ultimum. Our data suggest that chitinase might not be a unique lytic enzyme in controlling S. cerevisiae, M. grisea, P. capsici, and P. ultimum. R. solani, S. sclerotiorum. Also, suicide vector pEX18GM might be explored as a potential tool for gene deletions in L. enzymogenes, which will facilitate the molecular study of mechanisms of biological control in L. enzymogenes.
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