ArticlePharmaceutical research2012
Biodistribution studies of nanoparticles using fluorescence imaging: a qualitative or quantitative method?
Article in Pharmaceutical research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Mechanisms and determinants of mRNALipid Nanoparticle biodistribution: From pharmacokinetics to clinical translation.Bioengineering & translational medicine · 2026Review
- Systematic evaluation of magnetic particle imaging quantification strategies in biologically relevant scenarios using anatomically correct 3D printed mouse phantoms.Physics in medicine and biology · 2026Article
- Ultrasmall Gold Nanoparticles as "Three-in-One" Enzyme-Mimicking Nanocatalysts for Combined Sonodynamic/Catalytic Therapy in Breast Cancer.ACS applied materials & interfaces · 2025Article
- Pharmacokinetic Profiling of Unlabeled Magnetic Nanoparticles Using Magnetic Particle Imaging as a Novel Cold Tracer Assay.Nano letters · 2024Article
- The elasticity of silicone-stabilized liposomes has no impact on their in vivo behavior.Journal of nanobiotechnology · 2024Article
- Quantitative Pharmacokinetics Reveal Impact of Lipid Composition on Microbubble and Nanoprogeny Shell Fate.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- ROS-responsive PEGylated ferrocene polymer nanoparticles with improved stability for tumor-selective chemotherapy and imaging.Materials today. Bio · 2023Article
- Application of magnetic particle imaging to evaluate nanoparticle fate in rodent joints.Journal of controlled release : official journal of the Controlled Release Society · 2023Article
- Radiolabelling small and biomolecules for tracking and monitoring.RSC advances · 2022Review
- Hyperspectral dark field optical microscopy for orientational imaging of a single plasmonic nanocube using a physics-based learning method.Nanoscale advances · 2022Article
- The long-circulating effect of pegylated nanoparticles revisitedActa pharmaceutica Sinica. B · 2022Article
- Use of an antagonist of HMGB1 in mice affected by malignant mesothelioma: a preliminary ultrasound and optical imaging study.European radiology experimental · 2022Article
- Fluorophore Localization Determines the Results of Biodistribution of Core-Shell Nanocarriers.International journal of nanomedicine · 2022Article
- Radiolabeled RNA Nanoparticles for Highly Specific Targeting and Efficient Tumor Accumulation with FavorableMolecular pharmaceutics · 2021Article
- A method to improve quantitative radiotracing-based analysis of the in vivo biodistribution of drug carriers.Bioengineering & translational medicine · 2021Article
- Image-guided mathematical modeling for pharmacological evaluation of nanomaterials and monoclonal antibodies.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2020Review
- The clearance and biodistribution of magnetic composite nanoparticles in healthy and osteoarthritic rat knees.Journal of controlled release : official journal of the Controlled Release Society · 2020Article
- Concurrent fluorescence and volumetric optoacoustic tomography of nanoagent perfusion and bio-distribution in solid tumors.Biomedical optics express · 2019Article
- Systematic comparison of methods for determining the in vivo biodistribution of porous nanostructured injectable inorganic particles.Acta biomaterialia · 2019Article
- Rod-shape MSN@MoSTheranostics · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
purposeThe biodistribution of Lipid/Calcium/Phosphate (LCP) nanoparticles (NPs) in tumor-bearing mice was investigated using fluorescence imaging. A quantitative validation of this method was done by (3)H and (111)In labeling of the nanoparticles.
methodsThe biodistribution of LCP NPs containing oligonucleotides was investigated using three different probes: Texas-Red labeled oligonucleotides, (3)H-labeled oligonucleotides, and (111)In-labled calcium phosphate.
resultsA discrepancy was found between the radioactivity and the fluorescence signals. Signals from (3)H and (111)In exhibited very similar distribution patterns, suggesting that liver and spleen were the major accumulation sites. However, fluorescence imaging indicated that tumor accumulation was predominant. We further confirmed that the fluorescence signals in both liver and spleen were greatly attenuated compared with those in the tumor due to the intrinsic tissue absorption and scattering. Near-infrared (NIR) dye Cy5.5 also suffered from the same problem, in that the quantitative data from whole organs was dramatically affected by absorption and scattering properties of the tissue.
conclusionsCareful attention must be paid to the quantification and interpretation of fluorescence imaging measurements when comparing different tissues.
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