ArticleWorld journal of microbiology & biotechnology2012
Application of EMA-qPCR as a complementary tool for the detection and monitoring of Legionella in different water systems.
Article in World journal of microbiology & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 31 citations in OpenAlex.
- Antibiotic Resistance and Azithromycin Resistance Mechanism of Legionella pneumophila Serogroup 1 in China.Antimicrobial agents and chemotherapy · 2019Article
- Combined use of real-time PCR and nested sequence-based typing in survey of human Legionella infection.Epidemiology and infection · 2016Article
- Control of Legionella Contamination and Risk of Corrosion in Hospital Water Networks following Various Disinfection Procedures.Applied and environmental microbiology · 2016Article
- Population structure and minimum core genome typing of Legionella pneumophila.Scientific reports · 2016Article
- Prevalence and Molecular Characteristics of Waterborne Pathogen Legionella in Industrial Cooling Tower Environments.International journal of environmental research and public health · 2015Article
- Molecular characterization of viable Legionella spp. in cooling tower water samples by combined use of ethidium monoazide and PCR.Microbes and environments · 2015Article
- Detection of Legionella, L. pneumophila and Mycobacterium avium complex (MAC) along potable water distribution pipelines.International journal of environmental research and public health · 2014Article
- Distribution of sequence-based types of legionella pneumophila serogroup 1 strains isolated from cooling towers, hot springs, and potable water systems in China.Applied and environmental microbiology · 2014Article
- Management of the 2012 Legionella crisis in Quebec City: need for a better communication between resources and knowledge transfer.Frontiers in microbiology · 2014Article
- Identification and quantitative detection of Legionella spp. in various aquatic environments by real-time PCR assay.Environmental science and pollution research international · 2013Article
- High prevalence, genetic diversity and intracellular growth ability of Legionella in hot spring environments.PloS one · 2013Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legionella are prevalent in human-made water systems and cause legionellosis in humans. Conventional culturing and polymerase chain reaction (PCR) techniques are not sufficiently accurate for the quantitative analysis of live Legionella bacteria in water samples because of the presence of viable but nonculturable cells and dead cells. Here, we report a rapid detection method for viable Legionella that combines ethidium monoazide (EMA) with quantitative real-time PCR (qPCR) and apply this method to detect Legionella in a large number of water samples from different sources. Results yielded that samples treated with 5 μg/ml EMA for 10 min and subsequently exposed to light irradiation for 5 min were optimal for detecting Legionella. EMA treatment before qPCR could block the signal from approximately 4 log(10) of dead cells. When investigating environmental water samples, the percent-positive rate obtained by EMA-qPCR was significantly higher than conventional PCR and culture methods, and slightly lower than qPCR. The bacterial count of Legionella determined by EMA-qPCR were mostly greater than those determined by culture assays and lower than those determined by qPCR. Acceptable correlations were found between the EMA-qPCR and qPCR results for cooling towers, piped water and hot spring water samples (r = 0.849, P < 0.001) and also found between the EMA-qPCR and culture results for hot spring water samples (r = 0.698, P < 0.001). The results indicate that EMA-qPCR could be used as a complementary tool for the detection and monitoring of Legionella in water systems, especially in hot spring water samples.
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