ArticleWorld journal of microbiology & biotechnology2012
Anti-amoebic properties of a Malaysian marine sponge Aaptos sp. on Acanthamoeba castellanii.
Article in World journal of microbiology & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 37 citations in OpenAlex.
- Morphological and Chemical Changes in the Trophozoites and Cysts of Acanthamoeba Castellanii Induced by Camellia Sinensis Extracts.Acta parasitologica · 2025Article
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- Synthesis, anti-amoebic activity and molecular docking simulation of eugenol derivatives againstSaudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2023Article
- Amoebal Tubulin Cleavage Late during Infection Is a Characteristic Feature ofMicrobiology spectrum · 2022Article
- Anti-PeerJ · 2022Article
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- Ultrastructural Study ofPharmaceutics · 2020Article
- PLGA nanoparticles loaded with Gallic acid- a constituent of Leea indica against Acanthamoeba triangularis.Scientific reports · 2020Article
- Whole Organism Model to Study Molecular Mechanisms of Differentiation and Dedifferentiation.Biology · 2020Article
- Acanthamoeba in Southeast Asia - Overview and Challenges.The Korean journal of parasitology · 2019Review
- Toxic effects of selected proprietary dry eye drops on Acanthamoeba.Scientific reports · 2018Article
- Amoebicidal Activity of Caffeine and Maslinic Acid by the Induction of Programmed Cell Death in Acanthamoeba.Antimicrobial agents and chemotherapy · 2017Article
- Statins and voriconazole induce programmed cell death in Acanthamoeba castellanii.Antimicrobial agents and chemotherapy · 2015Article
- Anti-amoebic properties of carbonyl thiourea derivatives.Molecules (Basel, Switzerland) · 2014Article
- Composition of Archaea in seawater, sediment, and sponges in the Kepulauan Seribu reef system, Indonesia.Microbial ecology · 2014Article
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Authors and funding
7 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Crude methanol extracts of a marine sponge, Aaptos aaptos, collected from three different localities namely Kapas, Perhentian and Redang Islands, Terengganu, Malaysia, were tested in vitro on a pathogenic Acanthamoeba castellanii (IMR isolate) to examine their anti-amoebic potential. The examination of anti-Acanthamoebic activity of the extracts was conducted in 24 well plates for 72 h at 30 °C. All extracts possessed anti-amoebic activity with their IC(50) values ranging from 0.615 to 0.876 mg/mL. The effect of the methanol extracts on the surface morphology of A. castellanii was analysed under scanning electron microscopy. The ability of the extracts to disrupt the amoeba cell membrane was indicated by extensive cell's blebbing, changes in the surface morphology, reduced in cell size and with cystic appearance of extract-treated Acanthamoeba. Number of acanthapodia and food cup was also reduced in this Acanthamoeba. Morphological criteria of apoptosis in Acanthamoeba following treatment with the sponge's extracts was determined by acridine orange-propidium iodide staining and observed by fluorescence microscopy. By this technique, apoptotic and necrotic cells can be visualized and quantified. The genotoxic potential of the methanol extracts was performed by the alkaline comet assay. All methanol extracts used were significantly induced DNA damage compared to untreated Acanthamoeba by having high percentage of scores 1, 2, and 3 of the DNA damage. Results from cytotoxicity and genotoxicity studies carried out in the present study suggest that all methanol extracts of A. aaptos have anti-amoebic properties against A. castellanii.
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