ArticleWorld journal of microbiology & biotechnology2012
A comparison of fluorescent stains for the assessment of viability and metabolic activity of lactic acid bacteria.
Article in World journal of microbiology & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
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- Advancements in fluorescent labeling in assessing the probiotic adhesion capacity - A review.World journal of microbiology & biotechnology · 2025Review
- Method to Study the Survival Abilities of Foodborne Bacterial Pathogens Under Food Processing Conditions.Methods in molecular biology (Clifton, N.J.) · 2025Article
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- Development and Evaluation of a Thermosensitive In Situ Gel Formulation for Intravaginal Delivery ofPharmaceutics · 2022Article
- A critical analysis of research methods and experimental models to study irrigants and irrigation systems.International endodontic journal · 2022Review
- An antimicrobial peptide specifically active against Listeria monocytogenes is secreted by Bacillus pumilus SF214.BMC microbiology · 2022Article
- High-Pressure-Induced Sublethal Injuries of Food Pathogens-Microscopic Assessment.Foods (Basel, Switzerland) · 2021Article
- Temporal Changes of the Epiphytic Bacteria Community From the Marine Macroalga Ulva lactuca (Santa Marta, Colombian-Caribbean).Current microbiology · 2021Article
- Species Dependence of SYTO 9 Staining of Bacteria.Frontiers in microbiology · 2020Article
- Nondairy ice cream based on fermented yam (Food science & nutrition · 2019Article
- New Genes Involved in Mild Stress Response Identified by Transposon Mutagenesis inFrontiers in microbiology · 2018Article
- Shear treatment of starter culture medium improves separation behavior ofEngineering in life sciences · 2018Article
- Evolution of microbiological analytical methods for dairy industry needs.Frontiers in microbiology · 2014Review
- Aerobic metabolism and oxidative stress tolerance in the Lactobacillus plantarum group.World journal of microbiology & biotechnology · 2013Article
- Biofilm formation by Staphylococcus aureus and Salmonella spp. under mono and dual-species conditions and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactic acid bacteria (LAB) are used as starter or probiotic cultures in the food and pharmaceutical industries and, therefore, rapid and accurate methods for the detection of their viability are of practical relevance. In this study 10 LAB strains, belonging to the genera Enterococcus, Lactococcus, Leuconostoc, Lactobacillus, Streptococcus and Weissella, were subjected to heat and oxidative stresses and cell injury or death was assessed comparing different fluorescent probes (Syto 9; Propidium Iodide, PI; 4,6-diamidino-2-phenylindole, DAPI; 5,(6)-carboxyfluorescein diacetate, cFDA) to identify the stain combination which most reliably allowed the detection of live/metabolically active and dead cells. Protocols for specimen preparation and staining were optimized and a simple procedure for automated cell counts was developed using NIH ImageJ macros. Cysteine and semi-solid agar solution were efficiently used as anti-fading agent and mounting medium, respectively. The double staining cFDA-PI apparently offered the best and most versatile indication of both cell metabolic activity and membrane integrity. An excellent correlation between manual and automated cell counts for the majority of strain/stain combinations was found. This work provides a simple protocol for specimen preparation and staining based on the use of safe, easy to prepare and inexpensive reagents as compared to other methods. Additionally, the automated cell count procedure developed can be applied to several bacterial species and allows an increase in the number of experimental trials and the reproducibility and sensitivity of the analysis.
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Registered trials
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