ArticlePhotochemistry and photobiology
Sporicidal effects of high-intensity 405 nm visible light on endospore-forming bacteria.
Article in Photochemistry and photobiology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 96 citations in OpenAlex.
- The potential role of violet-blue light to preventing hospital acquired infections: a systematic review.Frontiers in public health · 2024Pooled it
- Selective bactericidal efficacy of 465-nm blue light phototherapy against standard and canine wound pathogens: AnVeterinary world · 2025Article
- The virucidal potential effects of violet-blue light on influenza D virus.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2025Article
- 405 nm violet-blue light inactivates hepatitis C cell culture virus (HCVcc) in ex vivo human platelet concentrates and plasma.Scientific reports · 2024Article
- Microbial reduction of prebagged human plasma using 405 nm light and its effects on coagulation factors.AMB Express · 2024Article
- The Preclinical Validation of 405 nm Light Parasiticidal Efficacy onMicroorganisms · 2024Article
- Safer school with near-UV technology: novel applications for environmental hygiene.Journal of environmental health science & engineering · 2023Article
- Efficacy of violet-blue light to inactive microbial growth.Scientific reports · 2022Article
- Low-power lasers on bacteria: stimulation, inhibition, or effectless?Lasers in medical science · 2021Review
- The viability of human cells irradiated with 470-nm light at various radiant energies in vitro.Lasers in medical science · 2021Article
- The virucidal effects of 405 nm visible light on SARS-CoV-2 and influenza A virus.Scientific reports · 2021Article
- Antimicrobial Blue Light versus Pathogenic Bacteria: Mechanism, Application in the Food Industry, Hurdle Technologies and Potential Resistance.Foods (Basel, Switzerland) · 2020Review
- Control Measures for SARS-CoV-2: A Review on Light-Based Inactivation of Single-Stranded RNA Viruses.Pathogens (Basel, Switzerland) · 2020Review
- Effects of an electric toothbrush combined with 3-color light-emitting diodes on antiplaque and bleeding control: a randomized controlled study.Journal of periodontal & implant science · 2020Article
- Bacterial cell growth is arrested by violet and blue, but not yellow light excitation during fluorescence microscopy.BMC molecular and cell biology · 2020Article
- Complete Inactivation of Blood Borne PathogenFrontiers in medicine · 2020Article
- Violet-Blue Light Arrays at 405 Nanometers Exert Enhanced Antimicrobial Activity for Photodisinfection of Monomicrobial Nosocomial Biofilms.Applied and environmental microbiology · 2019Article
- Electrically induced bacterial membrane-potential dynamics correspond to cellular proliferation capacity.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Non-ionizing 405 nm Light as a Potential Bactericidal Technology for Platelet Safety: Evaluation ofFrontiers in medicine · 2019Article
- Role of DNA Repair and Protective Components in Bacillus subtilis Spore Resistance to Inactivation by 400-nm-Wavelength Blue Light.Applied and environmental microbiology · 2018Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Resistance of bacterial endospores to treatments, including biocides, heat and radiation is a persistent problem. This study investigates the susceptibility of Bacillus and Clostridium endospores to 405 nm visible light, wavelengths which have been shown to induce inactivation of vegetative bacterial cells. Suspensions of B. cereus endospores were exposed to high-intensity 405 nm light generated from a light-emitting diode array and results demonstrate the induction of a sporicidal effect. Up to a 4-log(10) CFU mL(-1) reduction in spore population was achieved after exposure to a dose of 1.73 kJ cm(-2). Similar inactivation kinetics were demonstrated with B. subtilis, B. megaterium and C. difficile endospores. The doses required for inactivation of endospores were significantly higher than those required for inactivation of B. cereus and C. difficile vegetative cells, where ca 4-log(10) CFU mL(-1) reductions were achieved after exposure to doses of 108 and 48 J cm(-2), respectively. The significant increase in dose required for inactivation of endospores compared with vegetative cells is unsurprising due to the notorious resilience of these microbial structures. However, the demonstration that visible light of 405 nm can induce a bactericidal effect against endospores is significant, and could have potential for incorporation into decontamination methods for the removal of bacterial contamination including endospores.
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