ArticleGenetics and molecular biology2012
A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots.
Article in Genetics and molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Extraction of Proteins from Green Tissues of Plants and Phyllosphere.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Integrated Omic Approaches Reveal Molecular Mechanisms of Tolerance during Soybean andPlants (Basel, Switzerland) · 2022Article
- Article
- A comprehensive comparison of four methods for extracting lipids from Arabidopsis tissues.Plant methods · 2020Article
- Assessment and Refinement of Sample Preparation Methods for Deep and Quantitative Plant Proteome Profiling.Proteomics · 2018Article
- Differential proteome analysis during early somatic embryogenesis in Musa spp. AAA cv. Grand Naine.Plant cell reports · 2017Article
- Method developments to extract proteins from oil palm chromoplast for proteomic analysis.SpringerPlus · 2015Article
- Universal sample preparation method integrating trichloroacetic acid/acetone precipitation with phenol extraction for crop proteomic analysis.Nature protocols · 2014Article
- Transcriptional analysis of genes involved in nodulation in soybean roots inoculated with Bradyrhizobium japonicum strain CPAC 15.BMC genomics · 2013Article
- Proteomic insights into intra- and intercellular plant-bacteria symbiotic association during root nodule formation.Frontiers in plant science · 2013Article
- Proteomic Analysis of Soybean [Glycine max (L.) Merrill] Roots Inoculated with Bradyrhizobium japonicum Strain CPAC 15.Proteomics insights · 2013Article
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Authors and funding
5 authors.
Funding
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Abstract
Sample preparation is a critical step in two-dimensional gel electrophoresis (2-DE) of plant tissues. Here we describe a phenol/SDS procedure that, although greatly simplified, produced well-resolved and reproducible 2-DE profiles of protein extracts from soybean [Glycine max (L.) Merril] roots. Extractions were made in three replicates using both the original and simplified procedure. To evaluate the quality of the extracted proteins, ten spots were randomly selected and identified by mass spectrometry (MS). The 2-DE gels were equally well resolved, with no streaks or smears, and no significant differences were observed in protein yield, reproducibility, resolution or number of spots. Mass spectra of the ten selected spots were compared with database entries and allowed high-quality identification of proteins. The simplified protocol described here presents considerable savings of time and reagents without compromising the quality of 2-DE protein profiles and compatibility with MS analysis, and may facilitate the progress of proteomics studies of legume-rhizobia interactions.
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