Evidence map›Paper›PMID 22802591›Full record

ArticleThe Journal of physiology2012

Proliferation of myogenic stem cells in human skeletal muscle in response to low-load resistance training with blood flow restriction.

Jakob Lindberg Nielsen, Per Aagaard, Rune Dueholm Bech, Tobias Nygaard, Lars Grøndahl Hvid, Mathias Wernbom, Charlotte Suetta, Ulrik Frandsen

2 registry-linked trialsAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 122 papers, 8 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
122citing papers in PubMed, 8 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05396521 nacompletednot on this mapstarted 2022, after this paper: background citation

Utilizing Blood Flow Restriction Therapy Following ORIF Treated Distal Radius Fractures

TypeinterventionalSponsorCarilion ClinicRan2022 to 2023Enrolled4ConditionsDistal Radius FractureArmsBlood Flow Restriction, Standard of Care Physical Therapy
NCT05437770 nacompletednot on this mapstarted 2022, after this paper: background citation

Effects of Low-intensity Blood-flow Restricted Exercise Compared to Standard Rehabilitation in Patients With Knee Osteoarthritis - a Randomized Controlled Trial

TypeinterventionalSponsorBispebjerg HospitalRan2022 to 2025Enrolled96ConditionsOsteoarthritis, KneeArmsBFR (Blood-Flow Restricted exercise), Standard rehabilitation
3 · Its place in the literature

Who cites it

122 citing papers in PubMed, 8 syntheses or guidelines pooled it.

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62 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Jakob Lindberg NielsenInstitute for Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark.
Per Aagaard
Rune Dueholm Bech
Tobias Nygaard
Lars Grøndahl Hvid
Mathias Wernbom
Charlotte Suetta
Ulrik Frandsen

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Low-load resistance training with blood flow restriction has been shown to elicit substantial increases in muscle mass and muscle strength; however, the effect on myogenic stem cells (MSCs) and myonuclei number remains unexplored. Ten male subjects (22.8 ± 2.3 years)performed four sets of knee extensor exercise (20% 1RM) to concentric failure during bloodflow restriction (BFR) of the proximal thigh (100 mmHg), while eight work-matched controls(21.9 ± 3.0 years) trained without BFR (control, CON). Twenty-three training sessions were performed within 19 days. Maximal isometric knee extensor strength (MVC) was examined pre- and post-training, while muscle biopsies were obtained at baseline (Pre), after 8 days intervention(Mid8) and 3 (Post3) and 10 days (Post10) post training to examine changes in myofibre area (MFA), MSC and myonuclei number. MVC increased by 7.1% (Post5) and 10.6% (Post12)(P <0.001) with BFR training, while type I and II MFA increased by 38% (Mid8), 35 – 37%(Post3) and 31 – 32% (Post10) (P <0.001). MSCs per myofibre increased with BFR training from 0.10 ± 0.01 (Pre) to 0.38 ± 0.02 (Mid8), 0.36 ± 0.04 (Post3) and 0.25 ± 0.02 (Post10) (P <0.001). Likewise, myonuclei per myofibre increased from 2.49 ± 0.07 (Pre) to 3.30 ± 0.22(Mid8), 3.20 ± 0.16 (Post3) and 3.11 ± 0.11 (Post10), (P<0.01). Although MFA increased in CON at Mid8, it returned to baseline at Post3. No changes in MSC or myonuclei number were observed in CON. This study is the first to show that short-term low-load resistance exercise performed with partial blood flow restriction leads to marked proliferation of myogenic stem cells and resulting myonuclei addition in human skeletal muscle, which is accompanied by substantial myofibre hypertrophy.

Indexed as

Resistance TrainingAdult Stem CellsCell ProliferationExerciseHumansHypertrophyMaleMuscle, SkeletalMuscle StrengthMyoblasts, SkeletalRegional Blood FlowSatellite Cells, Skeletal MuscleYoung Adult

Identifiers

PMID22802591
PMCPMC3473290

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.