ArticleThe Journal of cell biology2012
PDZ interactions regulate rapid turnover of the scaffolding protein EBP50 in microvilli.
Article in The Journal of cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 48 citations in OpenAlex.
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- The Biological Relevance of NHERF1 Protein in Gynecological Tumors.Frontiers in oncology · 2022Review
- High-resolution dynamic mapping of the C. elegans intestinal brush border.Development (Cambridge, England) · 2021Article
- C-terminal phosphorylation modulates ERM-1 localization and dynamics to control cortical actin organization and support lumen formation duringDevelopment (Cambridge, England) · 2020Article
- Dynamic structure of the full-length scaffolding protein NHERF1 influences signaling complex assembly.The Journal of biological chemistry · 2019Article
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- Regulation of actin-based apical structures on epithelial cells.Journal of cell science · 2018Review
- The Phosphatase PTPL1 Is Required for PTEN-Mediated Regulation of Apical Membrane Size.Molecular and cellular biology · 2018Article
- A Tuba/Cdc42/Par6A complex is required to ensure singularity in apical domain formation during enterocyte polarization.PloS one · 2018Article
- Dynamic relocalization of NHERF1 mediates chemotactic migration of ovarian cancer cells toward lysophosphatidic acid stimulation.Experimental & molecular medicine · 2017Article
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- Controllable Activation of Nanoscale Dynamics in a Disordered Protein Alters Binding Kinetics.Journal of molecular biology · 2017Article
- Ras-activated RSK1 phosphorylates EBP50 to regulate its nuclear localization and promote cell proliferation.Oncotarget · 2016Article
- The function and dynamics of the apical scaffolding protein E3KARP are regulated by cell-cycle phosphorylation.Molecular biology of the cell · 2015Article
- The NHERF2 sequence adjacent and upstream of the ERM-binding domain affects NHERF2-ezrin binding and dexamethasone stimulated NHE3 activity.The Biochemical journal · 2015Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Scaffolding proteins containing PDZ (postsynaptic density 95/discs large/zonula occludens-1) domains are believed to provide relatively stable linkages between components of macromolecular complexes and in some cases to bridge to the actin cytoskeleton. The microvillar scaffolding protein EBP50 (ERM-binding phosphoprotein of 50 kD), consisting of two PDZ domains and an ezrin-binding site, retains specific proteins in microvilli and is necessary for microvillar biogenesis. Our analysis of the dynamics of microvillar proteins in vivo indicated that ezrin and microvillar membrane proteins had dynamics consistent with actin treadmilling and microvillar lifetimes. However, EBP50 was highly dynamic, turning over within seconds. EBP50 turnover was reduced by mutations that inactivate its PDZ domains and was enhanced by protein kinase C phosphorylation. Using a novel in vitro photoactivation fluorescence assay, the EBP50-ezrin interaction was shown to have a slow off-rate that was dramatically enhanced in a PDZ-regulated manner by addition of cell extract to near in vivo levels. Thus, the linking of relatively stable microvillar components can be mediated by surprisingly dynamic EBP50, a finding that may have important ramifications for other scaffolding proteins.
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