ArticleBiomaterials2012
Hydrogel macroporosity and the prolongation of transgene expression and the enhancement of angiogenesis.
Article in Biomaterials, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Antifibrotic hydrogel strategies for scarless tissue regeneration: mechanisms, design, and application.Bioactive materials · 2027Review
- Rational design matrix materials for organoid development and application in biomedicine.Regenerative biomaterials · 2025Review
- Acellular scaffold-based approach for in situ genetic engineering of host T-cells in solid tumor immunotherapy.Military Medical Research · 2024Article
- Biomembrane-Based Nanostructure- and Microstructure-Loaded Hydrogels for Promoting Chronic Wound Healing.International journal of nanomedicine · 2023Review
- Review
- Physical and mechanical cues affecting biomaterial-mediated plasmid DNA delivery: insights into non-viral delivery systems.Journal, genetic engineering & biotechnology · 2021Review
- Injectable, macroporous scaffolds for delivery of therapeutic genes to the injured spinal cord.APL bioengineering · 2021Article
- Injectable, Hyaluronic Acid-Based Scaffolds with Macroporous Architecture for Gene Delivery.Cellular and molecular bioengineering · 2019Article
- Biomaterial-Guided Gene Delivery for Musculoskeletal Tissue Repair.Tissue engineering. Part B, Reviews · 2017Review
- Localized lentivirus delivery via peptide interactions.Biotechnology and bioengineering · 2016Article
- Systematic evaluation of natural scaffolds in cutaneous wound healing.Journal of materials chemistry. B · 2015Article
- MicroRNA delivery for regenerative medicine.Advanced drug delivery reviews · 2015Review
- Porous hyaluronic acid hydrogels for localized nonviral DNA delivery in a diabetic wound healing model.Advanced healthcare materials · 2015Article
- Fabrication of cell-laden macroporous biodegradable hydrogels with tunable porosities and pore sizes.Tissue engineering. Part C, Methods · 2015Article
- Gene delivery strategies to promote spinal cord repair.Biomarker insights · 2015Review
- Heparin-chitosan nanoparticle functionalization of porous poly(ethylene glycol) hydrogels for localized lentivirus delivery of angiogenic factors.Biomaterials · 2014Article
- Cryotemplation for the Rapid Fabrication of Porous, Patternable Photopolymerized Hydrogels.Journal of materials chemistry. B · 2014Article
- Non-viral DNA delivery from porous hyaluronic acid hydrogels in mice.Biomaterials · 2014Article
- Matrix-based gene delivery for tissue repair.Current opinion in biotechnology · 2013Review
- A PLG/HAp composite scaffold for lentivirus delivery.Biomaterials · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The utility of hydrogels for regenerative medicine can be improved through localized gene delivery to enhance their bioactivity. However, current systems typically lead to low-level transgene expression located in host tissue surrounding the implant. Herein, we investigated the inclusion of macropores into hydrogels to facilitate cell ingrowth and enhance gene delivery within the macropores in vivo. Macropores were created within PEG hydrogels by gelation around gelatin microspheres, with gelatin subsequently dissolved by incubation at 37 °C. The macropores were interconnected, as evidenced by homogeneous cell seeding in vitro and complete cell infiltration in vivo. Lentivirus loaded within hydrogels following gelation retained its activity relative to the unencapsulated control virus. In vivo, macroporous PEG demonstrated sustained, elevated levels of transgene expression for 6 weeks, while hydrogels without macropores had transient expression. Transduced cells were located throughout the macroporous structure, while non-macroporous PEG hydrogels had transduction only in the adjacent host tissue. Delivery of lentivirus encoding for VEGF increased vascularization relative to the control, with vessels throughout the macropores of the hydrogel. The inclusion of macropores within the hydrogel to enhance cell infiltration enhances transduction and influences tissue development, which has implications for multiple regenerative medicine applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.