ArticleBiomaterials2012
The alignment and fusion assembly of adipose-derived stem cells on mechanically patterned matrices.
Article in Biomaterials, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
65 citing papers in PubMed, 157 citations in OpenAlex.
- Muscle-fiber-inspired nanofibrillar microbundles induce myogenic differentiation in human adipose-derived stem cells.Bioactive materials · 2026Article
- Microengineered Gradient Hydrogels for Mechanobiology.Advanced healthcare materials · 2026Review
- Myotube/Adipocyte Powder-Enriched Alginate-Zein Hydrogels Support Myotube Alignment for 3D Myoblast Culture.Foods (Basel, Switzerland) · 2026Article
- Contact stiffness governs cell mechanosensing through molecular clutches.Communications biology · 2025Article
- The influence of biomimetic conditions on neurogenic and neuroprotective properties of dedifferentiated fat cells.Stem cells (Dayton, Ohio) · 2025Article
- Article
- [Spatial Constraints of Rectangular Hydrogel Microgrooves Regulate the Morphology and Arrangement of Human Umbilical Vein Endothelial Cells].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2024Article
- Highly oriented hydrogels for tissue regeneration: design strategies, cellular mechanisms, and biomedical applications.Theranostics · 2024Review
- Durotaxis and negative durotaxis: where should cells go?Communications biology · 2023Review
- Skeletal muscle cells opto-stimulation by intramembrane molecular transducers.Communications biology · 2023Article
- Neuronal TIMP2 regulates hippocampus-dependent plasticity and extracellular matrix complexity.Molecular psychiatry · 2023Article
- Induced Pluripotent Stem Cells for Tissue-Engineered Skeletal Muscles.International journal of molecular sciences · 2023Review
- Insight into muscle stem cell regeneration and mechanobiology.Stem cell research & therapy · 2023Review
- Self-curling 3D oriented scaffolds from fish scales for skeletal muscle regeneration.Biomaterials research · 2022Article
- Article
- Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity.Biophysics and physicobiology · 2022Article
- The Golgi microtubules regulate single cell durotaxis.EMBO reports · 2021Article
- Recent Developments in Extracellular Matrix Remodeling for Fat Grafting.Frontiers in cell and developmental biology · 2021Review
- Effects of nanopatterned-surface dishes on chondrocyte growth and cell cycle progression.RSC advances · 2020Article
- Next Stage Approach to Tissue Engineering Skeletal Muscle.Bioengineering (Basel, Switzerland) · 2020Review
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Cell patterning is typically accomplished by selectively depositing proteins for cell adhesion only on patterned regions; however in tissues, cells are also influenced by mechanical stimuli, which can also result in patterned arrangements of cells. We developed a mechanically-patterned hydrogel to observe and compare it to extracellular matrix (ECM) ligand patterns to determine how to best regulate and improve cell type-specific behaviors. Ligand-based patterning on hydrogels was not robust over prolonged culture, but cells on mechanically-patterned hydrogels differentially sorted based on stiffness preference: myocytes and adipose-derived stem cells (ASCs) underwent stiffness-mediated migration, i.e. durotaxis, and remained on myogenic hydrogel regions. Myocytes developed aligned striations and fused on myogenic stripes of the mechanically-patterned hydrogel. ASCs aligned and underwent myogenesis, but their fusion rate increased, as did the number of cells fusing into a myotube as a result of their alignment. Conversely, neuronal cells did not exhibit durotaxis and could be seen on soft regions of the hydrogel for prolonged culture time. These results suggest that mechanically-patterned hydrogels could provide a platform to create tissue engineered, innervated micro-muscles of neural and muscle phenotypes juxtaposed next to each other in order better recreate a muscle niche.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.