ArticleJournal of tissue engineering2012
Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate.
Article in Journal of tissue engineering, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 23 citations in OpenAlex.
- Multicellular microtissues from fused ligament- and bone-cell spheroids relevant to enthesis repair.Materials today. Bio · 2026Article
- Mitochondria-Targeted Biophysical Priming of Autologous Biologics for Skin Regeneration and Wound Repair.International journal of molecular sciences · 2026Review
- Low-intensity pulsed ultrasound treatment in erectile dysfunction.Asian journal of andrology · 2025Review
- Emerging role of extracellular vesicles and exogenous stimuli in molecular mechanisms of peripheral nerve regeneration.Frontiers in cellular neuroscience · 2024Review
- Combining Visible Light and Non-Focused Ultrasound Significantly ReducesMicroorganisms · 2021Article
- Therapeutic Low-Intensity Ultrasound for Peripheral Nerve Regeneration - A Schwann Cell Perspective.Frontiers in cellular neuroscience · 2021Review
- Bioimaging: An Useful Tool to Monitor Differentiation of Human Embryonic Stem Cells into Chondrocytes.Annals of biomedical engineering · 2016Article
- Low-intensity pulsed ultrasound stimulation in different regions in the viability of myocutaneous flaps.Journal of therapeutic ultrasound · 2016Article
- Utilizing core-shell fibrous collagen-alginate hydrogel cell delivery system for bone tissue engineering.Tissue engineering. Part A · 2014Article
- Pulsed low-intensity ultrasound increases proliferation and extracelluar matrix production by human dermal fibroblasts in three-dimensional culture.Journal of tissue engineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, the effect of pulsed-low intensity ultrasound on cell proliferation, collagen production and glycosaminoglycan deposition by 3T3 fibroblasts encapsulated in alginate was evaluated. Hoechst 33258 assay for cell number, hydroxyproline assay for collagen content and dimethylamine blue assay for glycosaminoglycan content were performed on samples from cell cultures treated with pulsed-low intensity ultrasound and a control group. Pulsed-low intensity ultrasound shows no effect on cell proliferation, while collagen and glycosaminoglycan contents were consistently higher in the samples treated with pulsed-low intensity ultrasound, showing a statistically significant difference (p < 0.05) on day 10. Alcian blue staining showed that glycosaminoglycans were deposited around the cells in both groups. These results suggest that pulsed-low intensity ultrasound shows no effect on cell proliferation but has potential for inducing collagen and glycosaminoglycan production in cells cultured in alginate gels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.