ArticleGenetics2012
Integrity and function of the Saccharomyces cerevisiae spindle pole body depends on connections between the membrane proteins Ndc1, Rtn1, and Yop1.
Article in Genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Polo-like kinase Cdc5 orchestrates Cdk1 regulation via Swe1 and Mih1 during meiotic prophase I exit.Communications biology · 2026Article
- Spatiotemporal Dynamic Regulation of Organelles During Meiotic Development, Insights From Fungi.Frontiers in cell and developmental biology · 2022Article
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- A mutation in budding yeastmicroPublication biology · 2021Article
- High-Throughput Identification of Nuclear Envelope Protein Interactions inG3 (Bethesda, Md.) · 2020Article
- Yeast centrosome components form a noncanonical LINC complex at the nuclear envelope insertion site.The Journal of cell biology · 2019Article
- The Malleable Nature of the Budding Yeast Nuclear Envelope: Flares, Fusion, and Fenestrations.Journal of cellular physiology · 2016Review
- Mixing and matching nuclear envelope remodeling and spindle assembly strategies in the evolution of mitosis.Current opinion in cell biology · 2016Review
- Reticulons Regulate the ER Inheritance Block during ER Stress.Developmental cell · 2016Article
- Article
- Article
- Nuclear pore complex integrity requires Lnp1, a regulator of cortical endoplasmic reticulum.Molecular biology of the cell · 2015Article
- ER-associated retrograde SNAREs and the Dsl1 complex mediate an alternative, Sey1p-independent homotypic ER fusion pathway.Molecular biology of the cell · 2014Article
- Tts1, the fission yeast homologue of the TMEM33 family, functions in NE remodeling during mitosis.Molecular biology of the cell · 2014Article
- Breaching the nuclear envelope in development and disease.The Journal of cell biology · 2014Review
- The SUN protein Mps3 controls Ndc1 distribution and function on the nuclear membrane.The Journal of cell biology · 2014Article
- Article
- Exotic mitotic mechanisms.Open biology · 2012Review
- Genetic analysis of Mps3 SUN domain mutants in Saccharomyces cerevisiae reveals an interaction with the SUN-like protein Slp1.G3 (Bethesda, Md.) · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The nuclear envelope in Saccharomyces cerevisiae harbors two essential macromolecular protein assemblies: the nuclear pore complexes (NPCs) that enable nucleocytoplasmic transport, and the spindle pole bodies (SPBs) that mediate chromosome segregation. Previously, based on metazoan and budding yeast studies, we reported that reticulons and Yop1/DP1 play a role in the early steps of de novo NPC assembly. Here, we examined if Rtn1 and Yop1 are required for SPB function in S. cerevisiae. Electron microscopy of rtn1Δ yop1Δ cells revealed lobular abnormalities in SPB structure. Using an assay that monitors lateral expansion of the SPB central layer, we found that rtn1Δ yop1Δ SPBs had decreased connections to the NE compared to wild type, suggesting that SPBs are less stable in the NE. Furthermore, large budded rtn1Δ yop1Δ cells exhibited a high incidence of short mitotic spindles, which were frequently misoriented with respect to the mother-daughter axis. This correlated with cytoplasmic microtubule defects. We found that overexpression of the SPB insertion factors NDC1, MPS2, or BBP1 rescued the SPB defects observed in rtn1Δ yop1Δ cells. However, only overexpression of NDC1, which is also required for NPC biogenesis, rescued both the SPB and NPC associated defects. Rtn1 and Yop1 also physically interacted with Ndc1 and other NPC membrane proteins. We propose that NPC and SPB biogenesis are altered in cells lacking Rtn1 and Yop1 due to competition between these complexes for Ndc1, an essential common component of both NPCs and SPBs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.