ArticleGenetics2012
Rapid divergence of prolamin gene promoters of maize after gene amplification and dispersal.
Article in Genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 41 citations in OpenAlex.
- Meta-QTLs and candidate genes for kernel protein content in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- ZmMYB127 controls maize endosperm filling via dual-transcriptional regulation to improve grain yield and quality.Nature plants · 2026Article
- Identification of HSSP1 as a regulator of soybean protein content through QTL analysis and Soy-SPCC network.Plant biotechnology journal · 2025Article
- Central Roles of ZmNAC128 and ZmNAC130 in Nutrient Uptake and Storage during Maize Grain Filling.Genes · 2024Article
- The transcription factors ZmNAC128 and ZmNAC130 coordinate with Opaque2 to promote endosperm filling in maize.The Plant cell · 2023Article
- Efficient proteome-wide identification of transcription factors targeting Glu-1: A case study for functional validation of TaB3-2A1 in wheat.Plant biotechnology journal · 2023Article
- Nitrogen-dependent binding of the transcription factor PBF1 contributes to the balance of protein and carbohydrate storage in maize endosperm.The Plant cell · 2023Article
- Dynamic Transcriptome-Based Weighted Gene Co-expression Network Analysis Reveals Key Modules and Hub Genes Associated With the Structure and Nutrient Formation of Endosperm for Wax Corn.Frontiers in plant science · 2022Article
- The B3 domain-containing transcription factor ZmABI19 coordinates expression of key factors required for maize seed development and grain filling.The Plant cell · 2021Article
- Maize kernel development.Molecular breeding : new strategies in plant improvement · 2021Review
- The NAC Transcription Factors OsNAC20 and OsNAC26 Regulate Starch and Storage Protein Synthesis.Plant physiology · 2020Article
- Article
- The regulation of zein biosynthesis in maize endosperm.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Genome-wide analysis of wheat DNA-binding with one finger (Dof) transcription factor genes: evolutionary characteristics and diverse abiotic stress responses.BMC genomics · 2020Article
- NAC-type transcription factors regulate accumulation of starch and protein in maize seeds.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Development of Decreased-Gluten Wheat Enabled by Determination of the Genetic Basis ofPlant physiology · 2019Article
- Opaque-2 Regulates a Complex Gene Network Associated with Cell Differentiation and Storage Functions of Maize Endosperm.The Plant cell · 2018Article
- The ZmbZIP22 Transcription Factor Regulates 27-kD γ-Zein Gene Transcription during Maize Endosperm Development.The Plant cell · 2018Article
- OPAQUE11 Is a Central Hub of the Regulatory Network for Maize Endosperm Development and Nutrient Metabolism.The Plant cell · 2018Article
- Uniparental and transgressive expression of α-zeins in maize endosperm of o2 hybrid lines.PloS one · 2018Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Seeds have evolved to accommodate complicated processes like senescence, dormancy, and germination. Central to these is the storage of carbohydrates and proteins derived from sugars and amino acids synthesized during photosynthesis. In the grasses, the bulk of amino acids is stored in the prolamin superfamily that specifically accumulates in seed endosperm during senescence. Their promoters contain a conserved cis-element, called prolamin-box (P-box), recognized by the trans-activator P-box binding factor (PBF). Because of the lack of null mutants in all grass species, its physiological role in storage-protein gene expression has been elusive. In contrast, a null mutant of another endosperm-specific trans-activator Opaque2 (O2) has been shown to be required for the transcriptional activation of subsets of this superfamily by binding to the O2 box. Here, we used RNAi to knockdown Pbf expression and found that only 27-kDa γ- and 22-kDa α-zein gene expression were affected, whereas the level of other zeins remained unchanged. Still, transgenic seeds had an opaque seed phenotype. Combination of PbfRNAi and o2 resulted in further reduction of α-zein expression. We also tested the interaction of promoters and constitutively expressed PBF and O2. Whereas transgenic promoters could be activated, endogenous promoters appeared to be not accessible to transcriptional activation, presumably due to differential chromatin states. Although analysis of the methylation of binding sites of PBF and O2 correlated with the expression of endogenous 22-kDa α-zein promoters, a different mechanism seems to apply to the 27-kDa γ-zein promoter, which does not undergo methylation changes.
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