ArticleGenome biology and evolution2012
Vitamin B(12) synthesis and salvage pathways were acquired by horizontal gene transfer to the Thermotogales.
Article in Genome biology and evolution, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
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- Metabolic profiling analysis of the vitamin BMicrobiologyOpen · 2021Article
- Microbial and Genetic Resources for Cobalamin (Vitamin B12) Biosynthesis: From Ecosystems to Industrial Biotechnology.International journal of molecular sciences · 2021Review
- Functional traits of the gut microbiome correlated with host lipid content in a natural population ofBiology letters · 2020Article
- Genomic Insights of "Frontiers in microbiology · 2020Article
- Thermosipho spp. Immune System Differences Affect Variation in Genome Size and Geographical Distributions.Genome biology and evolution · 2018Article
- Article
- Microbial production of vitamin BMicrobial cell factories · 2017Review
- A Comprehensive Study of Cyanobacterial Morphological and Ecological Evolutionary Dynamics through Deep Geologic Time.PloS one · 2016Article
- Dynamics in microbial communities: unraveling mechanisms to identify principles.The ISME journal · 2015Article
- The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases.Nucleic acids research · 2014Article
- Thermotoga lettingae can salvage cobinamide to synthesize vitamin B12.Applied and environmental microbiology · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The availability of genome sequences of Thermotogales species from across the order allows an examination of the evolutionary origins of phenotypic characteristics in this lineage. Several studies have shown that the Thermotogales have acquired large numbers of genes from distantly related lineages, particularly Firmicutes and Archaea. Here, we report the finding that some Thermotogales acquired the ability to synthesize vitamin B(12) by acquiring the requisite genes from these distant lineages. Thermosipho species, uniquely among the Thermotogales, contain genes that encode the means to synthesize vitamin B(12) de novo from glutamate. These genes are split into two gene clusters: the corrinoid synthesis gene cluster, that is unique to the Thermosipho and the cobinamide salvage gene cluster. The corrinoid synthesis cluster was acquired from the Firmicutes lineage, whereas the salvage pathway is an amalgam of bacteria- and archaea-derived proteins. The cobinamide salvage gene cluster has a patchy distribution among Thermotogales species, and ancestral state reconstruction suggests that this pathway was present in the common Thermotogales ancestor. We show that Thermosipho africanus can grow in the absence of vitamin B(12), so its de novo pathway is functional. We detected vitamin B(12) in the extracts of T. africanus cells to verify the synthetic pathway. Genes in T. africanus with apparent B(12) riboswitches were found to be down-regulated in the presence of vitamin B(12) consistent with their roles in B(12) synthesis and cobinamide salvage.
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