ArticleEukaryotic cell2012
A detoxifying oxygen reductase in the anaerobic protozoan Entamoeba histolytica.
Article in Eukaryotic cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
- An iron-sulfur cluster as a new metal center in a flavodiiron protein.Protein science : a publication of the Protein Society · 2025Article
- Role of EhLINE1_ORF1p outside retrotransposition: Downregulation causes growth defects inJournal of biosciences · 2025Article
- Identification and characterization of archaeal-type FAD synthase as a novel tractable drug target from the parasitic protozoamSphere · 2024Article
- Episomal and chromosomal DNA replication and recombination inFrontiers in molecular biosciences · 2023Review
- Rubredoxin from the green sulfur bacterium Chlorobaculum tepidum donates a redox equivalent to the flavodiiron protein in an NAD(P)H dependent manner via ferredoxin-NAD(P)Archives of microbiology · 2021Article
- Rabeprazole inhibits several functions of Entamoeba histolytica related with its virulence.Parasitology research · 2020Article
- How the Anaerobic EnteropathogenmBio · 2020Article
- Biological and Bioinspired Inorganic N-N Bond-Forming Reactions.Chemical reviews · 2020Review
- The multidomain flavodiiron protein from Clostridium difficile 630 is an NADH:oxygen oxidoreductase.Scientific reports · 2018Article
- Divergent Transcriptional Responses to Physiological and Xenobiotic Stress in Giardia duodenalis.Antimicrobial agents and chemotherapy · 2016Article
- Novel Hydrogenosomes in the Microaerophilic Jakobid Stygiella incarcerata.Molecular biology and evolution · 2016Article
- The oxygen reduction pathway and heat shock stress response are both required for Entamoeba histolytica pathogenicity.Current genetics · 2016Review
- The dual function of flavodiiron proteins: oxygen and/or nitric oxide reductases.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2016Review
- Dioxygen and nitric oxide scavenging by Treponema denticola flavodiiron protein: a mechanistic paradigm for catalysis.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2015Article
- Flavodiiron oxygen reductase from Entamoeba histolytica: modulation of substrate preference by tyrosine 271 and lysine 53.The Journal of biological chemistry · 2014Article
- A diferrous-dinitrosyl intermediate in the N2O-generating pathway of a deflavinated flavo-diiron protein.Biochemistry · 2014Article
- The nitric oxide reductase mechanism of a flavo-diiron protein: identification of active-site intermediates and products.Journal of the American Chemical Society · 2014Article
- Novel functions of an iron-sulfur flavoprotein from Trichomonas vaginalis hydrogenosomes.Antimicrobial agents and chemotherapy · 2014Article
- Peroxynitrite and peroxiredoxin in the pathogenesis of experimental amebic liver abscess.BioMed research international · 2014Review
- Regulation of H2O2 stress-responsive genes through a novel transcription factor in the protozoan pathogen Entamoeba histolytica.The Journal of biological chemistry · 2013Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
We report the characterization of a bacterial-type oxygen reductase abundant in the cytoplasm of the anaerobic protozoan parasite Entamoeba histolytica. Upon host infection, E. histolytica is confronted with various oxygen tensions in the host intestine, as well as increased reactive oxygen and nitrogen species at the site of local tissue inflammation. Resistance to oxygen-derived stress thus plays an important role in the pathogenic potential of E. histolytica. The genome of E. histolytica has four genes that encode flavodiiron proteins, which are bacterial-type oxygen or nitric oxide reductases and were likely acquired by lateral gene transfer from prokaryotes. The EhFdp1 gene has higher expression in virulent than in nonvirulent Entamoeba strains and species, hinting that the response to oxidative stress may be one correlate of virulence potential. We demonstrate that EhFdp1 is abundantly expressed in the cytoplasm of E. histolytica and that the protein levels are markedly increased (up to ~5-fold) upon oxygen exposure. Additionally, we produced fully functional recombinant EhFdp1 and demonstrated that this enzyme is a specific and robust oxygen reductase but has poor nitric oxide reductase activity. This observation represents a new mechanism of oxygen resistance in the anaerobic protozoan pathogen E. histolytica.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.