ArticleTropical animal health and production2012
Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya.
Article in Tropical animal health and production, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Cryptosporidium infections in terrestrial ungulates with focus on livestock: a systematic review and meta-analysis.Parasites & vectors · 2019Pooled it
- Prevalence and molecular characterisation ofInternational journal for parasitology. Parasites and wildlife · 2026Article
- Article
- The prevalence and genetic characterisation ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Common occurrence of Cryptosporidium hominis in asymptomatic and symptomatic calves in France.PLoS neglected tropical diseases · 2018Article
- Estimating Cryptosporidium and Giardia disease burdens for children drinking untreated groundwater in a rural population in India.PLoS neglected tropical diseases · 2018Article
- Molecular detection and prevalence of Cryptosporidium spp. infections in two types of domestic farm animals in the Qinghai-Tibetan Plateau Area (QTPA) in China.Parasitology research · 2018Article
- Cryptosporidium and Giardia in Africa: current and future challenges.Parasites & vectors · 2017Review
- Diversity of Cryptosporidium species occurring in sheep and goat breeds reared in Poland.Parasitology research · 2017Article
- Article
- Prevalence of Cryptosporidia, Eimeria, Giardia, and Strongyloides in pre-weaned calves on smallholder dairy farms in Mukurwe-ini district, Kenya.Veterinary world · 2015Article
- A Multi-Locus Study for Detection of Cryptosporidium Species Isolated from Calves Population, Liverpool; UK.International journal of molecular and cellular medicine · 2014Article
- Prevalence and genetic characterization of Cryptosporidium in yaks in Qinghai Province of China.PloS one · 2013Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A total of 1,734 cattle faecal samples from 296 dairy-keeping households were collected from urban settings in Nairobi, Kenya. Modified Ziehl-Neelsen staining method and an immunofluorescence assay were used to identify those samples with Cryptosporidium oocyst infection. Oocysts from positive faecal samples were isolated by Sheather's sucrose flotation method and picked from the concentrate using cover slips. Genomic DNA was extracted from 124 of the faecal samples that were positive for Cryptosporidium and was used as template for nested PCR of the 18S rRNA gene. Twenty-five samples (20 %) were PCR-positive for Cryptosporidium, and 24 of the PCR products were successfully cloned and sequenced. Sequence and phylogenetic analysis identified 17 samples (68 %) as Cryptosporidium parvum-like, four samples (16 %) as Cryptosporidium ryanae, three samples (12 %) as Cryptosporidium andersoni and one sample (4 %) as Cryptosporidium hominis. To the best of our knowledge, this is the first genotyping study to report C. parvum-like, C. andersoni and C. hominis in cattle from Kenya. The results of this study show Cryptosporidium infections in calves and cattle may be potential zoonotic reservoirs of the parasite that infects humans.
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