ReviewThe Journal of membrane biology2012
Primary cultures of embryonic chick lens cells as a model system to study lens gap junctions and fiber cell differentiation.
Review in The Journal of membrane biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- Tonic ErbB signaling underlies TGFβ-induced activation of ERK and is required for lens cell epithelial to myofibroblast transition.Molecular biology of the cell · 2024Article
- ErbBs in Lens Cell Fibrosis and Secondary Cataract.Investigative ophthalmology & visual science · 2023Article
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- Article
- Methodologies to unlock the molecular expression and cellular structure of ocular lens epithelial cells.Frontiers in cell and developmental biology · 2022Article
- Insights into Bone Morphogenetic Protein-(BMP-) Signaling in Ocular Lens Biology and Pathology.Cells · 2021Review
- A functional map of genomic HIF1α-DNA complexes in the eye lens revealed through multiomics analysis.BMC genomics · 2021Article
- Development of a potent embryonic chick lens model for studying congenital cataracts in vivo.Communications biology · 2021Article
- Fibronectin regulates growth factor signaling and cell differentiation in primary lens cells.Journal of cell science · 2018Article
- Roles of TGFβ and FGF signals during growth and differentiation of mouse lens epithelial cell in vitro.Scientific reports · 2017Article
- Connexin 50 Functions as an Adhesive Molecule and Promotes Lens Cell Differentiation.Scientific reports · 2017Article
- Dual function of TGFβ in lens epithelial cell fate: implications for secondary cataract.Molecular biology of the cell · 2017Article
- The molecular mechanisms underlying lens fiber elongation.Experimental eye research · 2017Review
- Regulation of c-Maf and αA-Crystallin in Ocular Lens by Fibroblast Growth Factor Signaling.The Journal of biological chemistry · 2016Article
- Synergistic interaction between the fibroblast growth factor and bone morphogenetic protein signaling pathways in lens cells.Molecular biology of the cell · 2015Article
- Roles and regulation of lens epithelial cell connexins.FEBS letters · 2014Review
- Lens extrusion from Laminin alpha 1 mutant zebrafish.TheScientificWorldJournal · 2014Article
- Sciatic nerve regeneration using a nerve growth factor-containing fibrin glue membrane.Neural regeneration research · 2013Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
A major limitation in lens gap junction research has been the lack of experimentally tractable ex vivo systems to study the formation and regulation of fiber-type gap junctions. Although immortalized lens-derived cell lines are amenable to both gene transfection and siRNA-mediated knockdown, to our knowledge none are capable of undergoing appreciable epithelial-to-fiber differentiation. Lens central epithelial explants have the converse limitation. A key advance in the field was the development of a primary embryonic chick lens cell culture system by Drs. Sue Menko and Ross Johnson. Unlike central epithelial explants, these cultures also include cells from the peripheral (preequatorial and equatorial) epithelium, which is the most physiologically relevant population for the study of fiber-type gap junction formation. We have modified the Menko/Johnson system and refer to our cultures as dissociated cell-derived monolayer cultures (DCDMLs). We culture DCDMLs without serum to mimic the avascular lens environment and on laminin, the major matrix component of the lens capsule. Here, I review the features of the DCDML system and how we have used it to study lens gap junctions and fiber cell differentiation. Our results demonstrate the power of DCDMLs to generate new findings germane to the mammalian lens and how these cultures can be exploited to conduct experiments that would be impossible, prohibitively expensive and/or difficult to interpret using transgenic animals in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.