ArticleInternational journal of oncology2012
Analysis of a novel highly metastatic melanoma cell line identifies osteopontin as a new lymphangiogenic factor.
Article in International journal of oncology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Systemic rewiring of dendritic cells by melanoma-secreted midkine impairs immune surveillance and response to immune checkpoint blockade.Nature cancer · 2025Article
- Unraveling the molecular mechanisms of lymph node metastasis in ovarian cancer: focus on MEOX1.Journal of ovarian research · 2024Article
- Deletion of skeletal muscle Akt1/2 causes osteosarcopenia and reduces lifespan in mice.Nature communications · 2022Article
- The lymphatic vasculature: An active and dynamic player in cancer progression.Medicinal research reviews · 2022Review
- Live imaging of neolymphangiogenesis identifies acute antimetastatic roles of dsRNA mimics.EMBO molecular medicine · 2021Article
- Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism.Nature cancer · 2021Article
- Inactivation of EMILIN-1 by Proteolysis and Secretion in Small Extracellular Vesicles Favors Melanoma Progression and Metastasis.International journal of molecular sciences · 2021Article
- Midkine rewires the melanoma microenvironment toward a tolerogenic and immune-resistant state.Nature medicine · 2020Article
- Multipotent Adult Progenitor Cells Support Lymphatic Regeneration at Multiple Anatomical Levels during Wound Healing and Lymphedema.Scientific reports · 2018Article
- Article
- (Lymph)angiogenic influences on hematopoietic cells in acute myeloid leukemia.Experimental & molecular medicine · 2014Review
- Breast cancer as a systemic disease: a view of metastasis.Journal of internal medicine · 2013Review
- Proteinuria triggers renal lymphangiogenesis prior to the development of interstitial fibrosis.PloS one · 2012Article
- The miR-290-295 cluster suppresses autophagic cell death of melanoma cells.Scientific reports · 2012Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Tumor cell invasion and metastasis are hallmarks of malignancy. Despite recent advances in the understanding of lymphatic spread, the mechanisms by which tumors metastasize to sentinel/distant lymph nodes and beyond are poorly understood. To gain new insights into this complex process, we established highly metastatic melanoma cell lines by in vivo passaging the B16 parental cell line through the lymphatic system. In this study we characterized morphology, rate of cell proliferation, colony formation, migration, tumorigenicity, lymph flow, and capacities to induce tumor- and sentinel lymph node-lymphangiogenesis. Furthermore, microarray-based comparative analysis between parental and passaged cell lines was performed to identify specific gene expression profiles. The most differentially expressed gene was SPP (osteopontin), a secreted glycophosphoprotein which is known to be involved in cancer metastasis. Overexpression of osteopontin in B16 F1-variant was confirmed by western blot analysis and quantitative RT-PCR. Treatment of cultured lymphatic endothelial cells (LECs) with osteopontin promoted cell migration mediated by the integrin α9 pathway. Our results identify osteopontin as a novel lymphangiogenic factor.
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