ArticleJournal of virological methods2012
Comparison of fast-track diagnostics respiratory pathogens multiplex real-time RT-PCR assay with in-house singleplex assays for comprehensive detection of human respiratory viruses.
Article in Journal of virological methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
52 citing papers in PubMed, 2 syntheses or guidelines pooled it, 89 citations in OpenAlex.
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- Prevalence of respiratory viruses using polymerase chain reaction in children with wheezing, a systematic review and meta-analysis.PloS one · 2020Pooled it
- Clinical impact of rapid molecular detection of respiratory pathogens in patients with acute respiratory infection.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2018Trial
- Using Minimally Invasive Tissue Sampling and Determination of Cause of Death to Establish Etiologies of Community Respiratory Deaths Among Zambian Infants and Children.Journal of the Pediatric Infectious Diseases Society · 2025Article
- Burden of human metapneumovirus infections among children with acute respiratory tract infections attending a Tertiary Care Hospital, Kathmandu.BMC pediatrics · 2023Article
- Surveillance for Acute Respiratory Illnesses in Pediatric Chronic Care Facilities.Journal of the Pediatric Infectious Diseases Society · 2023Article
- Blood leukocyte transcriptional modules and differentially expressed genes associated with disease severity and age in COVID-19 patients.Scientific reports · 2023Article
- Lysophosphatidylcholine acyltransferase level predicts the severity and prognosis of patients with community-acquired pneumonia: a prospective multicenter study.Frontiers in immunology · 2023Observational
- Side-by-side comparison of parent vs. technician-collected respiratory swabs in low-income, multilingual, urban communities in the United States.BMC public health · 2022Article
- Distribution of respiratory viruses: Evaluation of multiplex PCR results of 3074 patients.Northern clinics of Istanbul · 2022Article
- Coronavirus Occurrence in the Household Influenza Vaccine Evaluation (HIVE) Cohort of Michigan Households: Reinfection Frequency and Serologic Responses to Seasonal and Severe Acute Respiratory Syndrome Coronaviruses.The Journal of infectious diseases · 2021Article
- Epidemiology of the Rhinovirus (RV) in African and Southeast Asian Children: A Case-Control Pneumonia Etiology Study.Viruses · 2021Article
- Comparison of performance between Fast Track Diagnostics Respiratory Kit and the CDC global reference laboratory for influenza rRT-PCR panel for detection of influenza A and influenza B.Influenza and other respiratory viruses · 2021Article
- The importance of viral load in the severity of acute bronchiolitis in hospitalized infants.Clinics (Sao Paulo, Brazil) · 2021Article
- Diagnostic Techniques for COVID-19: A Mini-review of Early Diagnostic Methods.Journal of analysis and testing · 2021Review
- Case Report: A Severe SARS-CoV-2 Infection in a Teenager With Angelman Syndrome.Frontiers in medicine · 2021Article
- Active surveillance for acute respiratory infections among pediatric long-term care facility staff.American journal of infection control · 2020Article
- Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Coronavirus Occurrence and Transmission Over 8 Years in the HIVE Cohort of Households in Michigan.The Journal of infectious diseases · 2020Article
- SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network.Memorias do Instituto Oswaldo Cruz · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fast-track Diagnostics respiratory pathogens (FTDRP) multiplex real-time RT-PCR assay was compared with in-house singleplex real-time RT-PCR assays for detection of 16 common respiratory viruses. The FTDRP assay correctly identified 26 diverse respiratory virus strains, 35 of 41 (85%) external quality assessment samples spiked with cultured virus and 232 of 263 (88%) archived respiratory specimens that tested positive for respiratory viruses by in-house assays. Of 308 prospectively tested respiratory specimens selected from children hospitalized with acute respiratory illness, 270 (87.7%) and 265 (86%) were positive by FTDRP and in-house assays for one or more viruses, respectively, with combined test results showing good concordance (K=0.812, 95% CI=0.786-0.838). Individual FTDRP assays for adenovirus, respiratory syncytial virus and rhinovirus showed the lowest comparative sensitivities with in-house assays, with most discrepancies occurring with specimens containing low virus loads and failed to detect some rhinovirus strains, even when abundant. The FTDRP enterovirus and human bocavirus assays appeared to be more sensitive than the in-house assays with some specimens. With the exceptions noted above, most FTDRP assays performed comparably with in-house assays for most viruses while offering enhanced throughput and easy integration by laboratories using conventional real-time PCR instrumentation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.