ArticlePloS one2012
Detection of Staphylococcus aureus delta-toxin production by whole-cell MALDI-TOF mass spectrometry.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 90 citations in OpenAlex.
- Pooled it
- Effect of selected microplastics on the development and spread of antibiotic resistance in bacteria.Folia microbiologica · 2026Article
- Impact of Light-Activated Nanocomposite with Erythrosine B onAntibiotics (Basel, Switzerland) · 2025Article
- MALDI-TOF MS Biomarkers for Methicillin-ResistantMetabolites · 2025Review
- Spectral Precision: Recent Advances in Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Pathogen Detection and Resistance Profiling.Microorganisms · 2025Review
- Contribution of MALDI-TOF mass spectrometry and machine learning including deep learning techniques for the detection of virulence factors of Clostridioides difficile strains.Microbial biotechnology · 2024Article
- Recent Advances in the Detection of Food Toxins Using Mass Spectrometry.Chemical research in toxicology · 2023Review
- Using Protein Fingerprinting for Identifying and Discriminating Methicillin ResistantDiagnostics (Basel, Switzerland) · 2023Article
- Genotypes ofToxins · 2022Article
- Diagnostic MALDI-TOF MS can differentiate between high and low toxicMicrobiology (Reading, England) · 2022Article
- Clonal Diversity, Antimicrobial Susceptibility and Presence of Genes Encoding Virulence Factors in Staphylococcus aureus Strains Isolated from Cut Wound Infections.Current microbiology · 2022Article
- The role of pathogens in diabetes pathogenesis and the potential of immunoproteomics as a diagnostic and prognostic tool.Frontiers in microbiology · 2022Review
- Correlations of Host and Bacterial Characteristics with Clinical Parameters and Survival inJournal of clinical medicine · 2021Article
- MALDI-TOF Mass Spectroscopy Applications in Clinical Microbiology.Advances in pharmacological and pharmaceutical sciences · 2021Review
- Insufficient repeatability and reproducibility of MALDI-TOF MS-based identification of MRSA.Folia microbiologica · 2020Article
- Review
- Concurrent Local Delivery of Diflunisal Limits Bone Destruction but Fails To Improve Systemic Vancomycin Efficacy during Staphylococcus aureus Osteomyelitis.Antimicrobial agents and chemotherapy · 2020Article
- Determination of methicillin-resistantClinical mass spectrometry (Del Mar, Calif.) · 2020Article
- Article
- Biofilm Formation byJournal of clinical medicine · 2019Article
Corrections and comments
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Authors and funding
19 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the present study was to detect the Staphylococcus aureus delta-toxin using Whole-Cell (WC) Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry (MS), correlate delta-toxin expression with accessory gene regulator (agr) status, and assess the prevalence of agr deficiency in clinical isolates with and without resistance to methicillin and glycopeptides. The position of the delta-toxin peak in the mass spectrum was identified using purified delta-toxin and isogenic wild type and mutant strains for agr-rnaIII, which encodes delta-toxin. Correlation between delta-toxin production and agr RNAIII expression was assessed by northern blotting. A series of 168 consecutive clinical isolates and 23 unrelated glycopeptide-intermediate S. aureus strains (GISA/heterogeneous GISA) were then tested by WC-MALDI-TOF MS. The delta-toxin peak was detected at 3005±5 Thomson, as expected for the naturally formylated delta toxin, or at 3035±5 Thomson for its G10S variant. Multivariate analysis showed that chronicity of S. aureus infection and glycopeptide resistance were significantly associated with delta-toxin deficiency (p = 0.048; CI 95%: 1.01-10.24; p = 0.023; CI 95%: 1.20-12.76, respectively). In conclusion, the S. aureus delta-toxin was identified in the WC-MALDI-TOF MS spectrum generated during routine identification procedures. Consequently, agr status can potentially predict infectious complications and rationalise application of novel virulence factor-based therapies.
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Registered trials
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